足球资讯网

COVID-19 呼应——咱们努力于在这场大风行病时代为迷信界供给撑持。领会更多

双光栅可进步仪器机能和检测矫捷性

SpectraMax® M 系列多功效微孔读板机(酶标仪)可测定紫外光 (UV) 和可见光光接收、荧光、发光、荧光偏振、时辰分辩荧光 (TRF) 和均相时辰分辩荧光 (HTRF)。规范功效包罗比色皿插口、1 nm 增量的光谱扫描,和单次多达 6 个波长的读取功效。这些功效壮大的酶标仪(读板机)已进入从南极洲到国际空间站的各个尝试室中。凭仗绝佳的试剂、考证东西和业内一流的 SoftMax® Pro 软件,它们可供给不变的机能。

  • 检测矫捷性

    检测矫捷性

    体系接纳双光栅作为单色器,是以您可以或许或许轻松找到适合的激起和发射波长,完成与公用单功效酶标仪(读板机)相媲美的检测机能。

  • 专利的 PathCheck 光程主动校订功效

    更切确的光接收测定

    操纵专利的 PathCheck® 传感器手艺来丈量样品深度,且不受温度的影响。它主动将光接收读数规范化至 1 cm,从而无需绘制规范曲线。

  • 周全的酶标仪(读板机)考证

    增添通量

    可与咱们的 StakMax® 微孔板堆板机轻松整合,以完成无人值守主动化。该堆板机可承载多达 50 块孔板并可设置装备摆设条形码阅读器。

SpectraMax m5e 多功效微孔读板机(酶标仪)

SpectraMax M5e 多功效微孔读板机(酶标仪)

特色

  • 专利优化功效

    SpectraMax M5 读板机的专利 AutoPMT 优化功效可根据各个样品孔的浓度来调理荧光检测器,并将原始数据规范化,从而增添检测的静态规模。

  • IQ/OQ/PM 办事

    经由历程咱们的 IQ/OQ/PM 办事可证实 GLP 或 GMP 情况下的 Molecular Devices 微孔读板机(酶标仪)及格,该办事以合规情势保管办事文档,并可长途停止拜候。

  • 考证东西

    与操纵多个平台来搜集和阐发数据比拟,考证东西的扩大套件可将考证本钱和时辰削减 50%。

  • 合规软件

    SoftMax Pro GxP 软件将数据收罗和阐发处置打算推行至遵守 GMP、GLP 和 21 CFR 第 11 局部请求的合规尝试室以完成电子记实的宁静性。

经考证的 GxP 处置打算可确保数据完全性和合规性

咱们为 GMP/GLP 尝试室供给一整套颠末考证的合规性处置打算,可以或许或许赞助您疾速、自傲地成立合规尝试室。

  • 一流的微孔板读板机和洗板机可撑持您的一切尝试须要
  • IQ/OQ/PM 办事以数字化和合规格局保管仪器记实
  • 软件装置办事可考证并记实所需的组件是不是按操纵规范装置
  • 软件考证办事撑持美国食物药品监视办理局 (FDA) 21 CFR Part 11 指南
  • 接纳可追溯的考证板来查验您的微孔板读板机机能,赞助您取得靠得住成果
合用于 GMP-GLP 尝试室的 GxP 合规性处置打算

 

SpectraMax M 系列多功效微孔读板机(酶标仪)的操纵

  • 光接收

    光接收

    领会有关光接收检测的一切信息 – 它若何运作、若何对它停止测定,和若何操纵它计较浓度。咱们还供给有关罕见光接收检测的操纵,包罗 ELISA、核酸和卵白定量,和微生物发展的信息。

    领会更多信息 

    细胞活气

    细胞活气

    可经由历程检测细胞膜完全性或细胞内酶活性等参数来评价细胞的活气。在微孔板读板机(酶标仪)上,可操纵荧光试剂来检测这些参数。比方,咱们操纵一种非细胞膜穿透性的红色染料和膜透性的活细胞绿色染料做为染色东西。由于一般细胞膜为非通透性的,红色染料仅能穿透细胞膜受损的死细胞或将死的细胞,与其 DNA 连系发生红色的荧光;而绿色的活细胞染料,仅在代谢酶有活性的活细胞中才会收回荧光,是以可以或许或许辨别死细胞和活细胞。

    阅读操纵申明 

  • ELISA

    ELISA

    酶联免疫吸附检测 (ELISA) 凡是以微孔板情势用于测定特定卵白的含量,且大多时辰均经由历程可见光波长规模内的吸光度来取得其成果。化学发光和荧光 ELISA 情势具备更高的活络度,可对少许待检测物资停止切肯定量。

    领会更多信息 

    荧光

    荧光

    领会有关荧光检测的一切信息 – 它是甚么、它若何运作,和若何操纵仪器测定样本荧光强度。另外,还涵盖了良多荧光检测的操纵,包罗细胞活性、G 卵白偶联受体活性,和荧光核酸定量等。

    领会更多信息  

  • 荧光偏振 (FP)

    荧光偏振

    荧光偏振 (FP) 是一项普遍用于监测不时变更的连系事务的手艺。其可用于评价包罗卵白-抗体连系和 DNA 杂交在内的生物份子彼此感化和酶活性,合用于根本钻研和高通量挑选。

    领会更多信息  

    荧光卵白检测

    荧光卵白检测

    荧光卵白作为用于检测生物体内各类反映的无力东西深受大师爱好。除维多利亚多管发光水母中的绿色荧光卵白 (GFP) 以外,此刻有良多其余来自水母和礁珊瑚等物种的荧光卵白可供操纵。这些卵白可在各类细胞和生物体中抒发,并在此中被用于监测良多细胞生物学历程,包罗卵白质分解和转位、基因引诱,和停止细胞谱系阐发。

    阅读操纵申明 

  • 发光

    发光

    领会发光检测 – 它是甚么、它若何运作,和发光检测与其余检测体例比拟的上风。咱们还供给关头发光检测,包罗双荧光素酶报告基因、化学发光 ELISA、细胞毒性,和生物发光共振能量转移 (BRET)。

    领会更多信息  

    SNP 基因分型

    SNP 基因分型

    基因分型是一种经由历程检测个别的 DNA 序列来阐发个别间基因差别的历程。单核苷酸多态性 (SNP) 是最罕见的遗传变异范例之一,包罗单个核苷酸在特定位点的渐变。经证实SNP 基因分型在检测差别物种的疾病相干渐变方面很是有效,是以,已开辟了良多 SNP 检测手艺。

    阅读操纵申明 

  • TRF、TR-FRET 和 HTRF

    时辰分辩

    领会有关时辰分辩荧光的一切信息 – TRF 和 TR-FRET,包罗 HTRF 检测。咱们将供给操纵资本以辅佐您停止钻研,包罗激酶检测、细胞旌旗灯号转导通路、卵白间彼此感化、细胞毒性等。

    领会更多信息  

SpectraMax M 系列多功效微孔板读板机(酶标仪)的手艺参数和可选设置装备摆设

 

*操纵最低的设置和速率读取。

SpectraMax M 系列多功效微孔读板机(酶标仪)的资本

报告内容
视频和搜集钻研会
SpectraMax m5e 多功效微孔读板机(酶标仪)

SpectraMax M5e 多功效微孔读板机(酶标仪)

  • Number of Citations*: 18,100

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Jan 15, 2012
    Publication Name: European Journal of Pharmacology

    Baicalein is one of the major flavonoids obtained from the Scutellaria root. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria.… View more

     is one of the major  obtained from the  root. Previous pharmacological studies found that baicalein had  effects in animal models of . The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria. Firstly, we investigated the effects of baicalein on rotenone-induced toxicity in PC12 cells. The results showed that baicalein suppressed rotenone-induced , and inhibited the accumulation of reactive  species, ATP deficiency,  dissipation, and caspase-3/7 activation in a concentration-dependent manner, indicating that baicalein likely improved . Furthermore, we used isolated rat brain mitochondria to evaluate the effect of baicalein. Treatment with baicalein prevented rotenone-induced reactive oxidant species production, ATP deficiency and  in isolated brain mitochondria. Interestingly, exposure of isolated mitochondria to baicalein promoted mitochondrial active respiration. These results suggest that baicalein may be a mitochondria-targeted  and exerts neuroprotective effects on rotenone-induced . This study supports our previous research that baicalein possesses neuroprotective activity in vivo and it is worthy of further study.

    Contributors: Xiao-xiu Li, Guo-rong He, Xin Mu Bei Xu, Shuo Tian Xin Yu, Fan-rui Meng, Zhao-hong Xuan, Guan-huaDu  

  • Citation
    Dated: Jun 30, 2011
    Publication Name: Molecular and Cellular Biochemistry

    Tissue accumulation of homocysteine occurs in classical homocystinuria, a metabolic disease characterized biochemically by cystathionine β-synthase deficiency. Vascular manifestations such as myocardial infarction, cerebral thrombosis, hepatic steatosis, and pulmonary embolism are common in this disease and poorly understood. In this study, we… View more

    Tissue accumulation of homocysteine occurs in classical homocystinuria, a metabolic disease characterized biochemically by cystathionine β-synthase deficiency. Vascular manifestations such as myocardial infarction, cerebral thrombosis, hepatic steatosis, and pulmonary embolism are common in this disease and poorly understood. In this study, we investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative stress (thiobarbituric acid-reactive substances, protein carbonyl content, 2′,7′-dichlorofluorescein fluorescence assay, and total radical-trapping antioxidant potent) and activities of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in the rat lung. Reduced glutathione content and glucose 6-phosphate dehydrogenase activity, as well as nitrite levels, were also evaluated. Wistar rats received daily subcutaneous injections of Hcy (0.3–0.6 μmol/g body weight) from the 6th to the 28th days-of-age and the control group received saline. One and 12 h after the last injection, rats were killed and the lungs collected. Hyperhomocysteinemia increased lipid peroxidation and oxidative damage to protein, and disrupted antioxidant defenses (enzymatic and non-enzymatic) in the lung of rats, characterizing a reliable oxidative stress. In contrast, this amino acid did not alter nitrite levels. Our findings showed a consistent profile of oxidative stress in the lung of rats, elicited by homocysteine, which could explain, at least in part, the mechanisms involved in the lung damage that is present in some homocystinuric patients.

    Contributors: Aline A. da Cunha, Andréa G. K. Ferreira, Maira J. da Cunha, Carolina D. Pederzolli, Débora L. Becker, Juliana G. Coelho, Carlos S. Dutra-Filho & Angela T. S. Wyse  

  • Citation
    Dated: Feb 02, 2009
    Publication Name: Translational Research

    Chronic kidney disease (CKD) is associated with accelerated atherosclerosis and increased mortality from cardiovascular disease. CKD results in oxidative stress, inflammation, and high-density lipoprotein (HDL) deficiency, which work in concert to promote atherosclerosis. Normal HDL confers protection against atherosclerosis by inhibiting the… View more

    Chronic kidney disease (CKD) is associated with accelerated atherosclerosis and increased mortality from cardiovascular disease. CKD results in oxidative stress, inflammation, and high-density lipoprotein (HDL) deficiency, which work in concert to promote atherosclerosis. Normal HDL confers protection against atherosclerosis by inhibiting the oxidation of lipids and lipoproteins and by retrieving surplus cholesterol and phospholipids from lipid-laden cells in the artery wall for disposal in the liver (reverse cholesterol transport).

    Contributors: Hamid Moradi, Madeleine V.Pahl, Reza, Elahimehr, Nosratola D.Vaziri  

SpectraMax® M 系列多功效微孔读板机(酶标仪)

产物

货号

SpectraMax M2 多功效微孔读板机(酶标仪)

M2

SpectraMax M2E 多功效微孔读板机(酶标仪)

M2E

SpectraMax M3 多功效微孔板读板机(酶标仪)

M3

SpectraMax M4 多功效微孔板读板机(酶标仪)

M4

SpectraMax M5 多功效微孔读板机(酶标仪)

M5

SpectraMax M5E 多功效微孔读板机(酶标仪)

M5E

SpectraTest ABS2 光接收考证板

0200-6191

SpectraTest FL1 荧光考证板

0200-5060

SpectraTest LM1 化学发光考证板

0200-6186

SpectraDrop™ 超微量板根本版套装

0200-6262

SpectraDrop™ 超微量板 HTS 版套装

0200-6267

StakMax 堆板机

StakMax

酶标仪(读板机)货架

9000-0756

SpectraMax M 系列多功效微孔板读板机(酶标仪)的相干产物和办事