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主动移液功效可确保一块微孔板上多种检测前提下的分歧性

FlexStation® 3 多功效微孔读板机(酶标仪)可测定光接收、荧光强度、荧光偏振、化学发光和时辰分辩荧光。可编辑的移液功效适合于须要当即检测和主动化加样的生归天学和细胞检测。该款读板机(酶标仪)可利用移液体系从 96 孔或 384 孔板上额定转移 3 次检测试剂。

  • 知足更多检测前提

    知足更多检测前提

    自界说要打针到每一个孔中的试剂 ,可削减试剂耗损并可完成在单个微孔板上摸索更多的检测前提。

  • 主动化

    主动移液

    经由进程主动移液来进步检测品质并增添通量,尽可以或许的削减偏差,从而在尝试进程中和差别尝试之间供给更严酷的检测 CV。

  • 考证读板机(酶标仪)

    考证读板机(酶标仪)

    利用咱们的 PathCheck® 功效来停止液体转移考证,以量化移液器移液机能。将其与 SoftMax® Pro 软件和 IQ/OQ/PQ 考证打算搭配利用,以适合 FDA 21 CFR 第 11 局部的请求。

FlexStation 3 多功效微孔板读板机(酶标仪)

FlexStation 3 多功效微孔板读板机(酶标仪)

特色

  • 移液头

    内置 8 通道移液头或 16 通道移液头可经由进程将 96 或 384 孔化合物板中的试剂转移至检测板来增添检测阐发的矫捷性。

  • 优化的激起光

    双光栅设想赞助用户挑选适合的尝试激起光和发射光波长并且避免差别尝试间须要改换高贵滤光片的费事。

  • 微量利用

    SpectraDrop™ 超微量检测板供给合用于小体积测定的高通量处置打算,可延长样品制备时辰并增添 DNA、RNA 和卵白钻研通量。

  • 兼容主动化装备

    FlexStation 3微孔板读板机(酶标仪)可与各类主动化装备兼容。咱们主导“主动化体系供给商协作火伴打算”使得咱们的微孔板读板机(酶标仪)体系可以或许与一切天下抢先的主动化体系疾速、便利的停止整合。

经考证的 GxP 处置打算可确保数据完全性和合规性

咱们为 GMP/GLP 尝试室供给一整套颠末考证的合规性处置打算,可以或许赞助您疾速、自傲地成立合规尝试室。

  • 一流的微孔板读板机和洗板机可撑持您的一切尝试须要
  • IQ/OQ/PM 办事以数字化和合规格局保管仪器记实
  • 软件装置办事可考证并记实所需的组件是不是按操纵规范装置
  • 软件考证办事撑持美国食物药品监督办理局 (FDA) 21 CFR Part 11 指南
  • 接纳可追溯的考证板来查验您的微孔板读板机机能,赞助您取得靠得住成果
合用于 GMP-GLP 尝试室的 GxP 合规性处置打算

 

FlexStation 3 多功效微孔板读板机(酶标仪)的利用

  • 光接收

    光接收

    领会有关光接收检测的一切信息 – 它若何运作、若何对它停止测定,和若何利用它计较浓度。咱们还供给有关罕见光接收检测的利用,包罗 ELISA、核酸和卵白定量,和微生物发展的信息。

    领会更多信息 

    ELISA

    ELISA

    酶联免疫吸附检测 (ELISA) 凡是以微孔板情势用于测定特定卵白的含量,且大多时辰均经由进程可见光波长规模内的吸光度来取得其成果。化学发光和荧光 ELISA 情势具有更高的活络度,可对少许待检测物资停止精肯定量。

    领会更多信息 

  • 酶活性

    酶活性

    酶活性可利用显色底物来停止及时检测,在将显色底物增添至酶中时,它的色彩会产生变更,这类变更可在光接收微孔板读板机(酶标仪)上检测。利用具有移液处置功效的仪器可主动增添底物,同时可检测反映,如许就可以检测到最初期的反映局部,从而取得加倍切确的连系亲和力估值。

    阅读利用申明 

    疾速能源学钙流检测

    能源学钙流检测

    经由进程荧光染料测定细胞内钙的增添环境,从而监测 Gq 卵白偶联受体 (GPCR) 活化环境。钙敏传染料会被接收至细胞的胞质中。配体连系受体后,钙会被开释到细胞胞质中。该染料可连系细胞内的钙,随后可检测到荧光加强。

    阅读利用申明 

  • 荧光

    荧光

    领会有关荧光检测的一切信息 – 它是甚么、它若何运作,和若何利用仪器测定样本荧光强度。另外,还涵盖了很多荧光检测的利用,包罗细胞活性、G 卵白偶联受体活性,和荧光核酸定量等。

    领会更多信息  

    荧光偏振 (FP)

    荧光偏振

    荧光偏振 (FP) 是一项普遍用于监测不时变更的连系事务的手艺。其可用于评价包罗卵白-抗体连系和 DNA 杂交在内的生物份子彼此感化和酶活性,合用于根本钻研和高通量挑选。

    领会更多信息  

  • 发光

    发光

    领会发光检测 – 它是甚么、它若何运作,和发光检测与其余检测体例比拟的上风。咱们还供给关头发光检测,包罗双荧光素酶报告基因、化学发光 ELISA、细胞毒性,和生物发光共振能量转移 (BRET)。

    领会更多信息  

    报告基因检测

    SpectraMax DuoLuc 高分辩率报告基因检测

    报告基因检测使钻研者可以或许肯定基因是不是抒发和抒发程度若何。常被用于钻研旌旗灯号转导通路、基因调控、调理元件的布局等。一个报告基因载体包罗一个启动子和报告基因或方针基因和报告基因。比方萤火虫荧光素酶,该报告基因的抒发可被轻松的检测到。将该载体转染到哺乳植物细胞中,当方针基因在细胞中抒发时,报告基因也被抒发,利用响应的检测试剂便可对报告基因的抒发程度停止测定。

    阅读利用申明 

  • TRF、TR-FRET 和 HTRF

    时辰分辩

    领会有关时辰分辩荧光的一切信息 – TRF 和 TR-FRET,包罗 HTRF 检测。咱们将供给利用资本以辅佐您停止钻研,包罗激酶检测、细胞旌旗灯号转导通路、卵白间彼此感化、细胞毒性等。

    领会更多信息  

FlexStation 3 多功效微孔板读板机(酶标仪)的手艺参数和可选设置装备摆设

 

*利用最低的设置和速率读取。

FlexStation 3 多功效微孔板读板机(酶标仪)的资本

报告内容
视频和收集钻研会
微孔板读板机,从 ELISA 到 AlphaScreen

从 ELISA 到 AlphaScreen,利用多功效读板机来扩大检测利用

FlexStation 3 多功效微孔板读板机(酶标仪)

FlexStation 3 多功效微孔板读板机(酶标仪)

  • Number of Citations*: 6,700

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Jan 12, 2022
    Publication Name: Humana Press

    Many commercial organizations currently use the Fluorometric Imaging Plate Reader (FLIPR®: Molecular Devices, Sunnyvale, CA) to conduct highthroughput measurements of intracellular Ca2+ concentration (see Chapter 7 ), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications… View more

    Many commercial organizations currently use the Fluorometric Imaging Plate Reader (FLIPR®: Molecular Devices, Sunnyvale, CA) to conduct highthroughput measurements of intracellular Ca2+ concentration (see Chapter 7 ), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications, the primary limitation of FLIPR (i.e., its requirement for single wavelength fluorescent probes using visible light excitation) is not a significant issue. Indeed, visible light probes offer certain benefits over their ultraviolet (UV)-excited ratiometric counterparts, such as reduced sample autofluorescence and higher absorbance, thereby allowing relatively low concentrations of dye to be used. However, under certain circumstances researchers may prefer to conduct high-throughput experiments with ratiometric dyes, particularly when issues of dye leakage, photobleaching, or signal-to-noise ratio become a concern.

    Contributors: Ian C. B. Marshall, Izzy Boyfield, Shaun McNulty  

  • Citation
    Dated: Aug 17, 2011
    Publication Name: Jove Journal

    The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to… View more

    The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to monitor fluorescence changes of a variety of fluorescent reagents. For example, FlexStation 3 has been used to study the function of Ca2+-permeable ion channels and G-protein coupled receptors by measuring the changes of intracellular free Ca2+ levels. Transient receptor potential (TRP) channels are a large family of nonselective cation channels that play important roles in many physiological and pathophysiological functions. Most of the TRP channels are calcium permeable and induce calcium influx upon activation. In this video, we demonstrate the application of FlexStation 3 to study the pharmacological profile of the TRPA1 channel, a molecular sensor for numerous noxious stimuli. HEK293 cells transiently or stably expressing human TRPA1 channels, grown in 96-well plates, are loaded with a Ca2+-sensitive fluorescent dye, Fluo-4, and real-time fluorescence changes in these cells are measured before and during the application of a TRPA1 agonist using the FLEX mode of the FlexStation 3. The effect of a putative TRPA1 antagonist was also examined. Data are transferred from the SoftMax Pro software to construct concentration-response relationships of TRPA1 activators and inhibitors.

    Contributors: Jialie Luo, Yingmin Zhu, Michael X. Zhu, Hongzhen Hu  

  • Citation
    Dated: Dec 15, 2005
    Publication Name: Journal of Neuroscience Methods

    The FlexStation is a 96 or 384 fluorescent plate reader with the capability of adding solutions during readings; it therefore has the potential to provide high throughput analyses of the functional characteristics of neurotransmitter-gated ion channels that can be examined using changes in fluorescence. The 5-HT3 receptor is one such protein, as… View more

    The FlexStation is a 96 or 384 fluorescent plate reader with the capability of adding solutions during readings; it therefore has the potential to provide high throughput analyses of the functional characteristics of neurotransmitter-gated ion channels that can be examined using changes in fluorescence. The 5-HT3 receptor is one such protein, as its activation results in a change in membrane potential due to the opening of a Ca2+-permeant, cation-selective channel; it can therefore be studied using both Ca2+- and membrane potential-sensitive fluorescent dyes. Here we have used the FlexStation to examine the function of recombinant 5-HT3 receptors expressed in HEK293 cells using both these classes of dye. The results show that the pharmacological characteristics of the receptor obtained using the FlexStation is similar to those reported using other functional methods, although caution must be applied when using the membrane potential dye as large changes in membrane potential can yield inaccurate EC50s. Modifying the constituents of the buffer, however, so that the change in membrane potential was reduced, yielded EC50 values that were similar to previously reported data. We conclude that the FlexStation is a useful tool for high throughput studies when examining the function of neurotransmitter receptors that result in either a change in Ca2+ concentration or membrane potential.

    Contributors: Kerry L .Price, Sarah C.R. Lummis  

FlexStation® 3 系列多功效微孔板读板机(酶标仪)

产物 货号
具有 SoftMax Pro 软件的 FlexStation 3 微孔板读板机(酶标仪)包罗:FlexStation 3 根本体系, 1 年质保,涵盖部件和野生办事。**不包罗 8 通道或 16 通道移液器吸头, 该移液器吸头必须零丁采办,且必须在订购仪器时一路订购。 Flex3
SpectraDrop™ 超微量板根本版套装 0200-6262
SpectraDrop™ 超微量板 HTS 版套装 0200-6267
FlexStation 3 台式多功效酶标仪(读板机) IQ/OQ 考证打算 0112-0160

FlexStation 3 多功效微孔板读板机(酶标仪)的相干产物和办事