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多波长检测,无需滤光片

具备双光栅的 Gemini XPS 和 EM 微孔板读板机(酶标仪)为荧光检测尝试供给优良且矫捷的激起光和发射光设置。多点孔域扫描优化了细胞尝试的活络度。按照外部规范停止怪异的校准,可实现对照样本之间的绝对荧光单元 (RFU)。温度可以或许在室温到 45°C 之间不变调理,撑持监测温度敏理性反映。

  • 无需改换滤光片

    无需改换滤光片

    免除对确认、采办和改换滤光片的费事。双光栅供给 250 - 850nm 的激起和发射波长挑选。

  • 更精准地停止测定

    更精准地停止测定

    孔扫描可以或许反映从微孔板孔中间的单个点到全数构造培育孔中的多个点的荧光测定值。

  • 防止丧失高旌旗灯号

    防止丧失高旌旗灯号

    专利主动 PMT 优化体系可以或许防止丧失饱和的高旌旗灯号值,并找到精确的酶标仪(读板机)设置。

Gemini

Gemini

特色

  • 顶读功效

    从 6 到 384 孔板,撑持顶读情势的 Gemini XPS 微孔板读板机(酶标仪)可在起点、能源学、光谱扫描和孔扫描情势下检测各类样本的荧光。

  • 顶读和底读功效

    从 6 到 384 孔板,撑持顶读和底读情势的 Gemini EM 微孔板读板机(酶标仪)可在起点、能源学、光谱扫描和孔扫描情势下检测各类样本的荧光。

Gemini XPS 和 EM 微孔板读板机(酶标仪)的利用

Gemini XPS 和 EM 微孔板读板机(酶标仪)的手艺参数和可选设置装备摆设

 

*利用最低的设置和速率读取。

Gemini XPS 和 EM 微孔板读板机(酶标仪)的资本

  • Number of Citations*: 9,610

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: May 01, 2011
    Publication Name: Bentham Science Publishers

    Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have… View more

    Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have designed and developed a series of novel amino acid-substituted gemini surfactants with the general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine, glycyl-lysine and, lysyl-lysine). These compounds were synthesized and tested in rabbit epithelial cells using a model plasmid and a helper lipid. Plasmid/gemini/lipid (P/G/L) nanoparticles formulated using these novel compounds achieved higher gene expression than the nanoparticles containing the parent unsubstituted compound. In this study, we evaluated the cytotoxicity of P/G/L nanoparticles and explored the relationship between transfection efficiency/toxicity and their physicochemical characteristics (such as size, binding properties, etc.). An overall low toxicity is observed for all complexes with no significant difference among substituted and unsubstituted compounds. An interesting result revealed by the dye exclusion assay suggests a more balanced protection of the DNA by the glycine and glycyl-lysine substituted compounds. Thus, the higher transfection efficiency is attributed to the greater biocompatibility and flexibility of the amino acid/peptide-substituted gemini surfactants and demonstrates the feasibility of using amino acid-substituted gemini surfactants as gene carriers for the treatment of diseases affecting epithelial tissue.

    Contributors: Singh, Jagbir; Yang, Peng; Michel, Deborah; E. Verrall, Ronald; Foldvari, Marianna; Badea, Ildiko  

  • Citation
    Dated: Feb 24, 2010
    Publication Name: ASSAY and Drug Development Technologies

    The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced… View more

    The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced chemotaxis may be a fruitful approach for developing therapeutics in treating these diseases. Cells undergo cytoskeletal rearrangement prior to chemotaxis, and therefore actin polymerization can be used as a surrogate readout more proximal to chemokine receptor activation than chemotaxis. Conventionally, actin polymerization is measured by fluorescence microscopy or flow cytometry, which are either of low throughput or in need of special instruments. We developed a 96-well actin polymerization assay that can process 1,000 to 1,500 samples a day. This assay uses a standard laboratory fluorescence microplate reader as the detection instrument and was optimized for various experimental conditions such as cell density, actin filament staining reagent, staining buffer, and cell culture conditions. We demonstrate that this actin polymerization assay in 96-well format exhibits the expected pharmacology for human CXCR5 and is suitable as a primary functional assay to screen neutralizing scFv in crude bacterial peri-preps and a secondary assay for small compound collections.

    Contributors: Jennifer Alley, Laird Bloom, Marion Kasaian, Huilan Gao, Gabriel Berstein, James D. Clark, and Wenyan Miao  

  • Citation
    Dated: May 09, 2005
    Publication Name: Journal of Materials Chemistry

    A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor… View more

    A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor cellular oxygen uptake and their ability to overcome a number of problems associated with oxygen sensing in biological samples was assessed. Macromolecular probes proved sufficient in a number of cases, particularly where spectral solutions can resolve the interferences. However where physical interactions cause interference the added protection of the polymer in the particle based probes was required.

    Contributors: Ciara O'Donovan, James Hynes, Dmitri Yashunski and Dmitri B. Papkovsky  

Gemini™ XPS 酶标仪(读板机)

产物

货号

Gemini XPS 微孔板读板机(荧光酶标仪) XPS
SpectraTest FL1 荧光考证板 0200-5060
SpectraDrop™ 超微量检测板根本套装 0200-6262
SpectraDrop™ 超微量板 HTS 版套装 0200-6267
StakMax 堆板机 StakMax
微孔板读板机(酶标仪)架 9000-0756

Gemini™ EM 酶标仪(读板机)

产物

货号

Gemini EM 微孔板读板机(荧光酶标仪) EM
SpectraTest FL1 荧光考证板 0200-5060
SpectraDrop™ 超微量检测板根本套装 0200-6262
SpectraDrop™ 超微量检测板 HTS 套装 0200-6267
微孔板读板机(酶标仪)架 9000-0756

Gemini XPS 和 EM 微孔读板机(酶标仪)的相干产物和办事