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具有样品追踪和孔板处置功效的微生物克隆遴选体系

QPix® 400 系列微生物克隆遴选体系连系了智能成像阐发和精准主动化,可对较大的文库停止疾速而高效的遴选。每小时可以或许或许或许挑取 3000 个克隆,它将简化您的使命流程。除微生物遴选外,体系还可主动履行多种样品制备和孔板处置,比方将菌液涂布到琼脂上。经由历程各类数据追踪和检测东西,QPix 软件可简化对庞杂和反复流程的节制和办理。

  • 宽度

    辨认特定表型的克隆

    QPix 微生物克隆遴选体系撑持各类差别的微生物和多种遴选体例,包罗荧光强度、蓝白斑遴选、巨细、克隆间距和抑菌圈。

  • 遴选

    高效挑取克隆

    微生物特同性挑针和琼脂厚度探测器可确保高效的挑取。体系具有 >98% 的挑取效力,确保靠得住的无看管运转。

  • 无菌

    坚持无菌状况

    具有浩繁灭菌功效,包罗用紫外灯对仪器外部消毒、和挑针的洗濯和卤素灯枯燥。

特色

  • 微生物特同性挑针

    差别外形和挑取地区的挑针可尽可以或许或许进步大肠杆菌、噬菌体和酵母菌的挑取效力。涂布公用针可确保将液体培育物平均散布在琼脂上。

  • 多种成像形式

    可操纵白光、荧光和色彩按照事后指定的参数来遴选克隆。滤光片的操纵可完成蓝白斑遴选等操纵。

  • 涂布和划线

    可在 30 分钟内主动涂布和划线 96 个样品,完成更长时候的无看管运转。

  • 复制、点样和重排

    主动孔板处置和追踪简化了下流检测和样品办理。QPix 微生物克隆遴选体系可供给矫捷的孔板复制、重排和点样功效。

  • 琼脂厚度探测器

    超声琼脂厚度探测器可检测因加样量的变更而发生的厚度差,从而进步挑取效力。

  • 可扩大主动化选项*

    QPix HT 型号是一个具有模块化平台并兼容机器臂的处置打算。进步前辈使命流程工程处置打算团队可量身打造撑持各类定制办事的微生物克隆遴选体系。

*价钱、托付时候和手艺参数将按照两边约定的手艺请求而有所差别。可以或许或许会按照处置打算请求调剂规范机能。

  • 信息图表

    信息图表

    检查主动微生物克隆遴选体系若何加速微生物钻研的历程。

  • 信息图表

    信息图表

    检查主动微生物克隆遴选体系若何加速分解生物学钻研的历程。

QPix 400 系列微生物克隆遴选体系的操纵

  • 抗生素抑菌圈

    QPix 软件在抗生素范畴的操纵

    产抗生素菌株的有用性可以或许或许经由历程细菌板上发生的通明圈巨细来测定。QPix 软件让您可以或许或许或许疾速辨认、摆列和挑取发生通明圈的微生物克隆。智能图象阐发可以或许或许或许测定板内的通明圈,并按照克隆巨细、光晕直径和圆度停止排序。

    生物燃料

    在 QPix 微生物克隆遴选体系中检测生物燃料

    生物柴油是最超卓的替换动力资本之一,它是一种由三酰基甘油构成且富含能量的便携式燃料。经由历程产脂微生物体系来生发生物柴油常须要遴选数千个克隆,阐发方式包罗经由历程二喹啉甲酸 (BCA) 检测、光密度丈量和蔼相色谱阐发等。QPix 微生物克隆遴选体系可主动履行克隆挑取使命(一个吃力且易犯错的历程),从而有用延长找到适合候选克隆的时候。

    阅读操纵申明 

  • 蓝白斑遴选

    QPix 400 与蓝白斑遴选

    经由历程拔出克隆化基因来对含有重组质粒的细菌转化株停止遴选是份子克隆中是必不可少的步骤。一种称为“蓝白斑遴选”的比色报告方式可完成按照色彩来轻松辨认重组和非重组克隆。QPix 微生物克隆遴选体系可供给一种特地用于操纵白光成像来停止蓝白斑比色遴选的主动化处置打算,以高效监测转化效力。其余比色方式,如“红白斑遴选”也可在体系上实行。

    阅读操纵申明 

    DNA 测序

    QPix与 DNA 测序

    测序即读取 DNA 份子内腺嘌呤 (A)、鸟嘌呤 (G)、胞嘧啶 (C) 和胸腺嘧啶 (T) 核苷酸简直切挨次。鸟枪法测序是将 DNA 朋分成数个含一千个碱基对的片断,而后将其亚克隆到圆形质粒,再转化到细菌中。主动化克隆挑取对进步测序的通量和质粒纯化相当首要。QPix 微生物克隆遴选体系以靠得住性和精确度著称,曾在人类基因组打算中被很多测序中间操纵。疫苗开辟等很多钻研范畴中仍在接纳传统的测序手艺。

    阅读操纵申明 

  • 噬菌体展现

    QPix微生物克隆遴选体系与噬菌体展现手艺

    噬菌体展现是一种可完成卵白质、多肽或 DNA 与靶卵白彼此感化钻研的手艺。这类份子东西可操纵噬菌体来显现病毒外壳外表的靶卵白,同时融会编码病毒外壳内靶卵白的 DNA,从而完成高亲和性连系物的发明。可显现功效的噬菌体可被遴选以用于与肽或卵白文库高通量连系。QPix 微生物克隆遴选体系可在噬菌体展现使命流程中完成主动接种、涂布、划线和挑取。

    领会更多信息 

    卵白质退化

    微生物克隆遴选体系与卵白质退化

    卵白质退化描画卵白质外形、功效和构成随时候推移而发生的变更。卵白质的定向退化经证明是转变和改良大份子活性的一种有用战略,可用于产业、钻研和医治操纵。因为具有多个荧光滤光片,该体系可与各类荧光克隆载体兼容。这使 QPix 微生物克隆遴选体系可以或许或许或许在钻研卵白质折叠、酶退化和卵白定位时显现个别克隆的怪异信息。这包罗寻觅转化标记和遴选渐变子。

QPix 400 系列微生物克隆遴选体系的手艺参数和可选设置装备摆设

QPix 400 系列微生物克隆遴选体系的资本

  • 信息图表

    信息图表

    检查主动微生物克隆遴选体系若何加速微生物钻研的历程。

  • 信息图表

    信息图表

    检查主动微生物克隆遴选体系若何加速分解生物学钻研的历程。

  • 宣扬页

    宣扬页

    分解生物学是一门跨学科的迷信,有可以或许或许影响包罗新型医治剂和疫苗的发生、植物…等在内的学术和行业操纵

  • 宣扬页

    宣扬页

    范围在生物学中成为一个愈来愈首要的身分。扩大某个历程的才能可增添或人更等闲和更有用发明......的机遇

  • 产物手册

    彩页

    QPix™ 体系在对人类基因组(人类基因组打算)停止排序的这场比赛中,因较高的机能和靠得住性而博得了杰出的名誉,并持续撑持......

  • 电子书合集

    电子书合集

    Accelerate vaccine development

    加速疫苗开辟

    与近代史上的任什么时候候比拟,免疫学此刻成为此中一个最首要的钻研范畴。单克隆抗体 (mAb) 作为一种潜伏医治,人们仍然对它坚持着稠密的乐趣......

    Download eBook

    下载电子书

  • 宣扬页

    宣扬页

    一个涵盖 QPix 机头系列的长处、差别的针功效和洗濯槽体系的长处的一页式文档。

  • 宣扬页

    宣扬页

    1985 年,George P. Smith 发了然一种将方针卵白的外源基因拔出噬菌体外壳卵白基因的方式,并初次对噬菌体展现停止了描写。该…

  • 产物手册

    彩页

    检查差别的定制可以或许或许性以知足您的特别使命流程请求

  • 产物手册

    彩页

    Molecular Devices 供给的产物和怪异处置打算操纵成像和智能图象阐发来撑持根本钻研、制药和生物医治开辟......

  • 客户冲破性停顿

    客户冲破性停顿

    经由历程供给完全的操纵法式接口 QPix 菌落遴选仪软件,本案例钻研凸起夸大了高等工程使命流程处置打算。本案例钻研凸起夸大了高等......

  • 客户冲破性停顿

    客户冲破性停顿

    阅读本案例钻研,以领会 DNA 构建体若何供给细胞功效,如编程干细胞在个别化医疗、发生可检测疾病的细菌和......方面的操纵

  • 迷信海报

    迷信海报

    检查海报以领会 QPix 400 系列,从菌落遴选到遴选,该系列都可完成全部使命流程的主动化,从而能延长时候线并进步全体......

  • 迷信海报

    迷信海报

    下载 PDF 文件以检查对 QPix 400 系列微生物克隆遴选体系的信息,它具有遴选性遴选罕见克隆的功效。

  • 迷信海报

    迷信海报

    摸索若何经由历程高尼罗红荧光强度停止克隆遴选,以在低级遴选中找到所需的高脂菌株。

  • 产物单页

    产物单页

    领会 QPix 主动化微生物克隆遴选体系若何知足各类差别微生物使命流程的钻研须要。

  • 操纵申明

    操纵文章

    Fluorescent Bacterial Colony Selection Using QPix 400 Systems

    操纵带有荧光报告基因的载体,可以或许或许使遴选含有与方针基因连系的质粒的细菌转化株变得更轻松。荧光…

    Read Application Note

  • 操纵申明

    操纵文章

    Easy picking with the QPix 400: multiple selection modalities, wide range of microorganisms

    经由历程庞杂的算法、具有自界说遴选规范且易于操纵的软件和微生物特定算法和附件,QPix 400 系列的微生物克隆遴选体系…

    Read Application Note

  • 操纵申明

    操纵文章

    High-throughput screening and selection of high lipid producers for biofuels

    高通量遴选脂类高产菌株用于生物燃料

    生发生物燃料的首要瓶颈题目之一是完成疾速靠得住的优良菌株辨认,而经由历程对终究功效的客观、功效性检测可完成此类辨认…

    Read Application Note

    阅读操纵申明

  • 操纵申明

    操纵文章

    Synthetic metagenomics: converting digital information back to biology

    美国动力部结合基因组钻研院 (DoE JGI) 的钻研职员已开辟出基于高通量遴选手艺的分解宏基因组学流程,以…

    Read Application Note

  • 操纵申明

    操纵文章

    Expanded fluorescence screening using QPix 400 series

    在基于载体的份子克隆中,细菌遴选是用于检测重组细菌的一种经常利用方式。它也可用于测定特定卵白抒发和…

    Read Application Note

报告内容
视频和收集钻研会
抗原/免疫原发明和优化

免疫学和疫苗开辟使命流程

SynBioBeta - QPix 小组会商2020

SynBioBeta - QPix 小组会商2020

噬菌斑挑取

噬菌斑挑取

宏基因组体系发育阐发

分解宏基因组学:将数字信息转换复生物学

QPix 400

QPix 400

  • Number of Citations*: 317

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Oct 28, 2014
    Publication Name: Front. Microbiol

    Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial… View more

    Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial activity by monocultures and pair-wise combinations of 146 phylogenetically different bacteria isolated from similar soil habitats. Growth responses of two human pathogenic model organisms, Escherichia coli WA321 and Staphylococcus aureus 533R4, were used to monitor antimicrobial activity. From all isolates, 33% showed antimicrobial activity only in monoculture and 42% showed activity only when tested in interactions. More bacterial isolates were active against S. aureus than against E. coli. The frequency of interaction-mediated induction of antimicrobial activity was 6% (154 interactions out of 2798) indicating that only a limited set of species combinations showed such activity. The screening revealed also interaction-mediated suppression of antimicrobial activity for 22% of all combinations tested. Whereas all patterns of antimicrobial activity (non-induced production, induced production and suppression) were seen for various bacterial classes, interaction-mediated induction of antimicrobial activity was more frequent for combinations of Flavobacteria and alpha- Proteobacteria. The results of our study give a first indication on the frequency of interference competitive interactions in natural soil bacterial communities which may forms a basis for selection of bacterial groups that are promising for the discovery of novel, cryptic antibiotics.

    Contributors: Olaf Tyc, Marlies van den Berg, Saskia Gerards, Johannes A. van Veen, Jos M. Raaijmakers, Wietse de Boer, and Paolina Garbeva  

  • Citation
    Dated: Mar 19, 2004
    Publication Name: The Journal of Biological Chemistry

    Thermotoga neapolitana 1,4-β-D-glucan glucohydrolase A preferentially hydrolyzes cello-oligomers, such as cellotetraose, releasing single glucose moieties from the reducing end of the cello-oligosaccharide chain. Using directed evolution techniques of error-prone PCR and mutant library screening, a variant glucan glucohydrolase has been isolated… View more

    Thermotoga neapolitana 1,4-β-D-glucan glucohydrolase A preferentially hydrolyzes cello-oligomers, such as cellotetraose, releasing single glucose moieties from the reducing end of the cello-oligosaccharide chain. Using directed evolution techniques of error-prone PCR and mutant library screening, a variant glucan glucohydrolase has been isolated that hydrolyzes the disaccharide, cellobiose, at a 31% greater rate than its wild type (WT) predecessor. The mutant library, expressed in Escherichia coli, was screened at 85 °C for increased hydrolysis of cellobiose, a native substrate rather than a chromogenic analog, using a continuous, thermostable coupled enzyme assay. The Vmax for the mutant was 108 ± 3 units mg-1, whereas that of the WT was 75 ± 2 units mg-1. The Km for both proteins was nearly the same. The kcat for the new enzyme increased by 31% and its catalytic efficiency (kcat/Km) for cellobiose also rose by 31% as compared with the parent. The nucleotide sequence of two positive clones and two null clones identified 11 single base shifts. The nucleotide transition in the most active clone caused an isoleucine to threonine amino acid substitution at position 170. Structural models for I170T and WT proteins were derived by sequence homology with Protein Data Bank code 1BGA from Paenibacillus polymyxa. Analysis of the WT and I170T model structures indicated that the substitution in the mutant enzyme repositioned the conserved catalytic residue Asn-163 and reconfigured entry to the active site.

    Contributors: James K. McCarthy, Aleksandra Uzelac, Diane F. Davis and Douglas E. Eveleigh  

  • Citation
    Dated: Aug 21, 2003
    Publication Name: the plant journal

    A genome‐wide transcription profiling of Arabidopsis upon genotoxic stress has been performed using a high‐density colony array (HDCA). The array was based on a library of 27 000 cDNA clones derived from Arabidopsis cells challenged with bleomycin plus mitomycin C. The array covers more than 10 000 individual genes (corresponding to at least 40%… View more

    A genome‐wide transcription profiling of Arabidopsis upon genotoxic stress has been performed using a high‐density colony array (HDCA). The array was based on a library of 27 000 cDNA clones derived from Arabidopsis cells challenged with bleomycin plus mitomycin C. The array covers more than 10 000 individual genes (corresponding to at least 40% of Arabidopsis genes). After hybridisation of the HDCA with labelled cDNA probes obtained from genotoxin‐treated (bleomycin plus mitomycin C, 6 h) and untreated seedlings, 39 genes revealed an increased and 24 genes a decreased expression among the 3200 highly expressed clones (representing approximately 1200 individual genes because of redundancy of the cDNA library). Of the 4900 clones with a low transcriptional level, the expression of 500 clones was found to be altered and 57 genes with increased and 22 genes with decreased expression were identified by sequence analysis of 135 identified clones. The HDCA results were validated by real‐time PCR analysis. For about 80% of genes (34 out of 42), alteration in expression was confirmed, indicating the reliability of the HDCA for transcription profiling. DNA damage and stress‐responsive genes encoding, for instance transcription factors (myb protein and WRKY1), the ribonucleotide reductase small subunit (RNR2), thymidine kinase (TK), an AAA‐type ATPase, the small subunit of a DNA polymerase and a calmodulin‐like protein were found to be strongly upregulated. Also, several genes involved in cell cycle regulation revealed significant alteration in transcription, as detected by real‐time PCR analysis, suggesting disturbance of cell cycle progression by mutagen treatment.

    Contributors: I‐Peng Chen, Urs Haehnel, Lothar Altschmied, Ingo Schubert, Holger Puchta  

QPix 400 系列微生物克隆遴选体系的相干产物和办事