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亲目睹证单克隆性

CloneSelect™ Single-Cell Printer™ 系列接纳近似喷墨打印机和一次性分派分手槽,暖和高效地接种单细胞。操纵明场高分辩率成像或可选荧光分选细胞,每一个单细胞分手捕获 5 张图象。证实单克隆性,进步使命效力,坚持并加强细胞活率,且避免穿插净化。

  • 图象

    收罗单细胞分手的证据

    在接种细胞时记实 5 张持续图象,以 96 或 384 孔板的情势供给间接的单克隆性图象证据。

  • 高效

    进步克隆构成率

    与传统体例比拟,在克隆构成率上可完成高达8倍的晋升。高效地将单细胞接种至 96 或 384 孔板中。接种一块96孔板只要约  5 分钟。

  • 挑选

    坚持细胞安康和无菌

    正如克隆发展尝试所见,经由进程暖和的分手坚持细胞活性,并操纵无菌的一次性单向分手槽避免穿插净化。

CloneSelect 高通量单细胞分手体系

特色

  • 图象-Gn

    图象证据

    每次分派单细胞时可捕获 5 张图象,从而供给可用于羁系文件中的单克隆性图象证据。

  • 图象-Gn

    荧光成像和分选

    按照活细胞荧光染料分选活细胞,富集细胞群以高效力地发生荧光报告基因,或分手所需的荧光 CRISPR 克隆。

  • 精确性-Gn

    微流控手艺

    无菌分手槽内含专有的硅微流控芯片,可天生包裹细胞的皮升液滴。仅将方针液滴接种到孔板中。

  • 无菌-Gn

    非标记成像和分选

    按照异质细胞群的巨细和形状分选细胞,每一个孔可挑选接种 1 至 100 个细胞。

  • 兼容一系列的细胞

    可分派各类百般的细胞范例,如 CHO、HEK293、L929、T 细胞和 B 细胞。细胞巨细从 5 到 40um 不等。

  • 无菌分手槽

    零丁包装的无菌分手槽旨在让细胞样本不与仪器打仗。一个分手槽可用于制备多块微孔板。

CloneSelect Single-Cell Printer 系列的操纵

  • 细胞株开辟

    针对重组卵白的细胞株株开辟

    细胞株开辟是制备生物制药份子(如单克隆抗体)进程中的关头步骤。该进程凡是从将编码方针卵白的 DNA 转染至宿主细胞起头,方针 DNA 会随机或定向整合至宿主细胞基因组中。挑选数以千计的克隆以辨别罕见的高产细胞是一个手动且耗时的进程。

    单克隆性

    细胞株开辟和单克隆性考证

    细胞株开辟和单克隆性考证是生发生物制药份子(如单克隆抗体)的进程中的关头步骤。在分手不变抒发方针卵白的单个活细胞后,可成立细胞株。此进程中的一个关头结点是记实单克隆性证据。单克隆性证据凡是都是图象情势,据此可天生单细胞图象并将其随附在羁系文件中。

    领会更多信息 

  • 单细胞基因组学

    单细胞基因组学

    单细胞分手依然是单细胞基因组学中一项有挑衅性的使命。现行体例贫乏证据证实阐发容器中唯一一个细胞。细胞在裂解前坚持其完全性很主要,以掩护其 DNA。

    另外,细胞在裂解前,应遭到尽可以或许或许少的应激,以掩护其 RNA 及其抒发程度。CloneSelect Single-Cell Printer 系列可很是暖和地分手细胞,从而确保高纯度和高活性。

    这为下流单细胞基因组阐发供给了最好根本。

    单细胞分选

    操纵 CloneSelect 单细胞分手体系停止单细胞分选

    细胞株开辟请求发明源于单细胞的克隆,它可以或许或许高程度地分歧地抒发方针医治卵白。此进程的第一步是分手活的单细胞。无限浓缩是一种依靠于统计几率的手艺,但很耗时。CloneSelect Single-Cell Printer 可以或许或许以尽可能进步细胞活性的体例来暖和分手单细胞,同时经由进程在每次单细胞分手时捕获的 5 张持续图象供给单克隆性的间接证据。

CloneSelect Single-Cell Printer 系列的手艺参数和可选设置装备摆设

 

** 用户操纵最好参数停止疾速分选时,在明场中可于 <10 分钟内和在荧光分选(仅 f.sight)中可于 <5 分钟内处置一块含 10 μm 珠悬浮液(溶于无菌滤过水中,浓度约为 5 x 105 至 1 x 106 个珠/ml)的细胞培育 96 孔板。

CloneSelect Single-Cell Printer 系列的资本

报告内容
视频和收集钻研会
开辟和成立不变细胞系

不变的细胞系开辟使命流程

抗原/免疫原发明和优化

免疫学和疫苗开辟使命流程

细胞系开辟使命流程

细胞株开辟的多种差别使命流程

f.Sight 与 FACS 和 LD 概述

f.Sight 与 FACS 和 LD 概述

敏捷辨认中和抗体

优化的使命流程可用于敏捷辨别中和抗体与病毒颗粒

CloneSelect 高通量单细胞分手体系

基于微流控手艺的单细胞

基于微流控手艺的单细胞分手使命流程,已针对效力、存活率和单克隆性考证停止优化

  • Number of Citations*: 5

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Nov 19, 2019
    Publication Name: Biotechnology

    During biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from… View more

    During biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from Berkeley Lights offers a strategy to eliminate these limitations through culturing, manipulating, and characterizing cells on custom nanofluidic chips via software‐controlled operations. However, explicit demonstration of this technology to provide high assurance of a single cell progenitor has not been reported. Here, a methodology that utilizes the Beacon instrument to ensure high levels of clonality is described. It is demonstrated that the Beacon platform can efficiently generate production cell lines with a superior clonality data package, detailed tracking, and minimal resources. A stringent in‐process quality control strategy is established to enable rapid verification of clonal origin, and the workflow is validated using representative Chinese hamster ovary‐derived cell lines stably expressing either green or red fluorescence protein. Under these conditions, a >99% assurance of clonal origin is achieved, which is comparable to existing imaging‐coupled fluorescence‐activated cell sorting seeding methods.

    Contributors: Kim Le, Christopher Tan, Huong Le, Jasmine Tat, Ewelina Zasadzinska, Jonathan Diep, Ryan Zastrow, Chun Chen, Jennitte Stevens  

  • Citation
    Dated: Jun 28, 2018
    Publication Name: Genetic Engineering & Biotechnology News

    Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice. View more

    Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice.

    Contributors: Patricia Fitzpatrick Dimond  

  • Citation
    Dated: Oct 28, 2017
    Publication Name: Genetic Engineering & Biotechnology News

    The increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company. View more

    The increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company.

    Contributors: Sarmad Al-Bassam, Steve Wiltgen  

CloneSelect 高通量单细胞分手体系

 

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产物 货号
CloneSelect Single-Cell Printer f.sight 仪器 CLONESELECT-F.SIGHT
CloneSelect Single-Cell Printer c.sight 仪器 CLONESELECT-C.SIGHT
CloneSelect Single-Cell Printer SCP 仪器 CLONESELECT-SCP

CloneSelect Single-Cell Printer 系列的相干产物和办事