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用于考证单克隆性的非标记成像处置打算

CloneSelect® 成像仪是一个高通量主动处置打算,可用于哺乳植物细胞的成像和阐发。经由进程多个时候点的成像跟踪可轻松地回溯源自单个细胞的克隆构成进程。主动收罗和阐发可供给切确、客观和分歧的成果。具体的成像和综合数据阐发可确保作出更好的决议打算。凭仗在 90 秒内对 96 孔板停止成像的才能,该细胞发展阐发体系将进步通量和效力。

  • 考证

    轻松考证单克隆性

    单克隆性报告功效可简化提交至羁系机构的撑持性材料的筹办任务。它将按照您遴选的参数主动天生报告。

  • 切确度

    精准检测细胞

    优化算法以切确地辨认细胞,并能处置差别的细胞范例和前提。高分辩率成像供给了单克隆性的切确度和可托度。

  • 高效

    在更短时候内遴选更多的克隆

    这款细胞发展阐发体系供给业内一流的成像时候,可在 90 秒内对 96 孔板停止成像。

特色

  • 成像情势遴选

    非标记明场可完成在不操纵无害荧光染料的环境下疾速收罗图象。自界说荧光成像可增添单克隆性的可托度。

  • 主动天生的数据和东西

    该软件主动计较会合度并天生发展曲线、热图和拼接图象。可随时候推移主动追踪每一个孔的测定值。

  • 各类孔板范例和细胞范例

    此细胞发展阐发体系兼容 6 至 384 孔板且可用于悬浮细胞和贴壁细胞,如 CHO、HEK、杂交瘤细胞、Per.C6®®、Jurkat、SupT1、H-4-II-E、MCF-7、JT29、DLD1 和 KB31。

  • 智能阐发

    该软件主动计较每一个成像时候点的会合度。主动天生可导出的发展曲线、拼接图象、总发展率和均匀速度。

  • 清楚简练的图象

    此细胞发展阐发体系具备可主动对焦的 4x 物镜。高分辩率 CCD 相机成像到达每像素 1.85 微米,即便在孔边缘等难以成像的地区也能完成精准的细胞检测。

  • 定制主动化选项*

    进步前辈任务流程工程处置打算团队供给各类定制办事,涵盖从机器臂孔板加载到整合液体任务站和培育箱的全主动任务站等各个方面。

*价钱、托付时候和手艺参数将按照两边约定的手艺请求而有所差别。可以或许或许会按照处置打算请求调剂规范机能。

  • 产物手册

    彩页

    CloneSelect Imager 细胞发展阐发体系可在更短的时候内天生客观、量化和分歧的成果,以降服与传统细胞会合度手艺有关的挑衅。

CloneSelect Imager 细胞发展阐发体系的操纵

  • 细胞株开辟

    针对重组卵白的细胞株株开辟

    细胞株开辟是制备生物制药份子(如单克隆抗体)进程中的关头步骤。该进程凡是从将编码方针卵白的 DNA 转染至宿主细胞起头,方针 DNA 会随机或定向整合至宿主细胞基因组中。遴选数以千计的克隆以辨别罕见的高产细胞是一个手动且耗时的进程。

    细胞活气

    细胞活气

    细胞株开辟便是发明源自单个细胞的克隆,其可不变高抒发方针医治性卵白。此进程中分手单个活细胞是关头的第一步。单细胞增殖构成克隆,随后可评价其方针医治卵白的产率。单细胞源性克隆的活性和发展速度可在 CloneSelect Imager 成像仪SpectraMax i3x 微孔板读板机和成像细胞计数仪上停止表征。

  • 克隆构成尝试

    克隆构成尝试

    软琼脂克隆构成尝试是一种传统且着名的手艺,用于表征细胞不依靠于贴壁而发展的才能,这是癌发生的标记。在半固体培育基等可增添克隆构成率的基质中接种后,细胞凡是会孵育在可以或许或许影响克隆发展的化合物中。CloneSelect Imager 细胞发展阐发体系可对每一个孔停止成像,并主动计较会合度、预算克隆数目和面积和追踪克隆的发展。

    非标记细胞迁徙

    CloneSelect Imager 细胞发展阐发体系上的非标记细胞迁徙

    划痕尝试在很多学科中都被用来钻研细胞迁徙,即细胞群的协同挪动。在微孔板中培育的单层细胞上制作一个手工或规范划痕。微孔板可针对化合物文库停止遴选,以钻研对细胞迁徙的影响。操纵这类方式可以或许或许钻研化合物或基因库的影响。CloneSelect Imager 细胞发展阐发体系可以或许或许疾速成像(每板 < 90 秒),并对细胞迁徙停止客观、主动阐发。

    阅读操纵申明 

  • 单克隆性

    单克隆性保障

    细胞株开辟和单克隆性考证是生发生物制药份子(如单克隆抗体)的进程中的关头步骤。在分手不变抒发方针卵白的单个活细胞后,可成立细胞株。此进程中的一个关头结点是记实单克隆性证据。单克隆性证据凡是都是图象情势,据此可天生单细胞图象并将其随附在羁系文件中。

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CloneSelect Imager 细胞发展阐发体系的手艺参数和可选设置装备摆设

CloneSelect Imager 的资本

报告内容
视频和收集钻研会
开辟和成立不变细胞系

不变的细胞系开辟任务流程

杂交瘤任务流程

杂交瘤任务流程

细胞系开辟任务流程

细胞株开辟的多种差别任务流程

敏捷辨认中和抗体

优化的任务流程可用于敏捷辨别中和抗体与病毒颗粒

CloneSelect Imager 细胞发展阐发体系视频

CloneSelect Imager 细胞发展阐发体系

G 卵白偶联受体细胞系遴选

操纵 ClonePix 2 来辨认和遴选 GPCR 细胞系

  • Number of Citations*: 72

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Merck Research Laboratory

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line… View more

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.

    Contributors: Shuangping Shi, Russ G.G. Condon, Liang Deng, Jason Saunders, Finn Hung, Yung-Shyeng Tsao, Zhong Liu  

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time… View more

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.

    Contributors: Wilson Lew, Anna Forsyth, John Philips, Alison Glaser, Natalia Lysaya  

  • Citation
    Dated: Jun 17, 2010
    Publication Name: BMC Biotechnology

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid,… View more

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.

    Contributors: Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk & Julian F Burke  

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