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具备主动克隆遴选功效的细胞株开辟处置打算

ClonePix® 2 哺乳植物细胞克隆遴选体系是一个全主动体系,可用于遴选在抗体发明和细胞株开辟中操纵的优良细胞克隆。对杂交瘤细胞、CHO 细胞和其余细胞范例成像并按照用户界说的参数停止遴选。周全集成孔板处置、条形码读取和克隆挑取功效。保管包罗图象在内的一切数据,以用于下流阐发。该遴选体系增添了找到罕见优良细胞株的几率并较着节流了时候和人力。

  • 高效

    在更短时候内遴选更多的克隆

    ClonePix 2 遴选体系要比休息麋集的无限浓缩法和 FACS 快 10 倍。咱们进步前辈的软件和集成式机器装备可完成天天 > 10,000 个克隆的遴选速率。

  • 遴选

    遴选具备所需特征的细胞

    按照卵白质产率、抗原特同性、细胞活性和已标记重组卵白的抒发程度来轻松遴选并挑取克隆。

  • 精确度

    精准挑取克隆

    挑取精度 < 1mm。机器挑取可下降克隆搅扰的危险。已挑取克隆的图象和数据一并保管。

ClonePix 2 高通量细胞克隆遴选体系

ClonePix 2

特色

  • 多种检测方式

    白光可检测并丈量克隆形状、尺寸和间距。荧光可唆使抒发程度和/或特同性。多达 5 种荧光滤光片可供操纵。

  • 坚持无菌性

    规范设置装备摆设含一系列灭菌功效和选项,包罗给仪器外部消毒的紫外灯,和挑针洗濯和卤素灯枯燥。

  • 集成储板体系

    包罗源板和终板的两个堆板架,每一个可包容 10 块板。

  • 团圆的克隆构成

    半固体培育基 CloneMediaTM 撑持将单细胞培育为团圆的克隆,并撑持轻松加样处置。该培育基可完成密度更高的克隆遴选。

  • 不含植物成份的培育基和试剂

    化学成份限制且不含植物成份的 CloneMedia 培育基经优化可增添产率,且在与 CloneDetectTM 检测试剂一起操纵时有助于显现所排泄的抗体。

  • 定制主动化选项*

    进步前辈任务流程工程处置打算团队可自界说单克隆体系并供给单克隆性综合考证等附加办事。

*价钱、托付时候和手艺参数将按照两边约定的手艺请求而有所差别。可以或许会按照处置打算请求调剂规范机能。

  • 产物手册

    彩页

    检查 PDF 文件以领会若何加速细胞株开辟并更快地完成您的方针。

ClonePix 2 哺乳植物细胞克隆遴选体系的操纵

  • 细胞株开辟

    针对重组卵白的细胞株株开辟

    细胞株开辟是制备生物制药份子(如单克隆抗体)进程中的关头步骤。该进程凡是从将编码方针卵白的 DNA 转染至宿主细胞起头,方针 DNA 会随机或定向整合至宿主细胞基因组中。遴选数以千计的克隆以辨别罕见的高产细胞是一个手动且耗时的进程。

    细胞外表抒发遴选

    细胞外表抒发遴选

    良多在细胞外表抒发的卵白都是用于发明和开辟生物医药的靶标。比方,G 卵白偶联受体 (GPCR) 是最大的细胞外表卵白种别,并且是约莫 40% 现有药物的靶标。从转染后的细胞池中发明和遴选细胞外表 GPCR 抒发增添的高代价克隆可以或许极具挑衅性。ClonePix 2 体系是可遴选大批细胞的一种主动化方式,它增添了发明罕见高亲和力杂交瘤细胞或高产细胞的几率。

  • 克隆产量遴选和滴度

    克隆产量遴选和滴度

    辨认优良克隆的一个主要步骤是测订单细胞来历克隆的抒发量。操纵传统方式遴选产率吃力且耗时,凡是包罗多个步骤,包罗从无限浓缩平分手单细胞以后再用 ELISA 停止滴度评价。ClonePix 2 体系将表型遴选、单细胞分手和产率遴选合为一个步骤,是以可较着延长遴选时候并增添候选物数目。

    杂交瘤细胞遴选

    杂交瘤细胞遴选

    抗体开辟凡是是指遴选和辨认能靶向特定表位以完成疾病诊治的单克隆抗体 (mAb)。发生单克隆抗体的一种经常操纵方式是将有丝割裂前的癌症细胞与脾脏中有丝割裂后和抒发终端抗体的 B 细胞融会。发生的融会细胞称为杂交瘤细胞,它具备发生单克隆抗体的上风,同时还可割裂再生。操纵 ClonePix 2 体系可主动遴选杂交瘤细胞的连系特同性或产率。

    领会更多信息 

  • 单克隆抗体发明

    抗原特同性遴选

    抗体开辟凡是是指遴选和辨认能靶向抗原份子以完成疾病诊治的特同性抗体。抗体的特同性取决于其连系表位(抗原份子上的怪异地区)的才能。医治性抗体凡是为单细胞源性单克隆抗体,可靶向抗原上的怪异表位地区。ClonePix 2 体系可从异源细胞群中主动遴选和疾速检测抗原特同性克隆。

    领会更多信息  

    单克隆性

    细胞株开辟和单克隆性考证是生发生物制药份子(如单克隆抗体)的进程中的关头步骤。在分手不变抒发方针卵白的单个活细胞后,可成立细胞株。此进程中的一个关头结点是记实单克隆性证据。单克隆性证据凡是都是图象情势,据此可天生单细胞图象并将其随附在羁系文件中。

    领会更多信息 

ClonePix 2 哺乳植物细胞克隆遴选体系的手艺参数和可选设置装备摆设

ClonePix 2 哺乳植物细胞克隆遴选体系的资本

  • 产物手册

    彩页

    检查 PDF 文件以领会若何加速细胞株开辟并更快地完成您的方针。

  • 产物手册

    彩页

    操纵转化细胞株开辟任务流程从头界说克隆遴选和遴选。

  • 宣扬页

    宣扬页

    再生医学可以或许为天下上某些最严重的疾病供给治愈方式。但在鞭策这些疗法方面,仍然存在良多妨碍......

  • 电子书合集

    电子书合集

    Accelerate vaccine development

    加速疫苗开辟

    与近代史上的任什么时候候比拟,免疫学此刻成为此中一个最主要的钻研范畴。单克隆抗体 (mAb) 作为一种潜伏医治,人们仍然对它坚持着稠密的乐趣......

    Download eBook

    下载电子书

  • 电子书合集

    电子书合集

    Optimize your high-value cell lines

    在曩昔的十年中,经由进程哺乳植物细胞来出产单克隆抗体 (mAb) 和重组卵白使得将医治卵白引入......风行临时

    Download eBook

  • 迷信海报

    迷信海报

    CloneSelect 成像仪体系可在更短的时候内天生客观、量化和分歧的成果,从而降服与传统细胞融会手艺有关的挑衅......

  • 迷信海报

    迷信海报

    在此海报中,咱们显现了标明 CloneMedia-PER.C6 撑持将细胞培育成可操纵 ClonePix......停止成像、排序和遴选的团圆菌落的数据

  • 迷信海报

    迷信海报

    此 PDF 文件切磋了基于在半固体培育基(源于单母细胞)中培育哺乳植物细胞的 ClonePix FL 手艺。

  • 迷信海报

    迷信海报

    发生耐受性较佳的疗法是 Octagene 的焦点合作力。血友病患者要毕生接管药物医治以避免出血。潜伏......的平常危险

  • 迷信海报

    迷信海报

    经由进程无限浓缩法、ring 型克隆或仅经由进程手动收罗菌落来分手候选哺乳植物细胞克隆是一个耗时、须要大批资本且本钱较高的进程......

  • 迷信海报

    迷信海报

    经由进程无限浓缩法、ring 型克隆或仅经由进程手动收罗菌落来分手哺乳植物细胞克隆是一个耗时、须要大批资本且本钱较高的进程......

  • 迷信海报

    迷信海报

    细胞培育手艺方面的停顿正鞭策新型遴选测定法的开辟,这些测定法使药物发明名目可以或许依靠于可停止高通量......的细胞程度检测

  • 迷信海报

    迷信海报

    遴选不变高产的细胞株是开辟和出产新卵白制剂的关头限速步骤。

  • 迷信海报

    迷信海报

    因为哺乳植物细胞在天生临床相干......方面具备出色的保真度,是以植物细胞培育手艺已被推到制药行业的前沿

  • 产物手册

    彩页

    Molecular Devices 供给的产物和怪异处置打算操纵成像和智能图象阐发来撑持根本钻研、制药和生物医治开辟......

  • 信息图表

    信息图表

    检查从 CHO-ori 到 CHO-K1SV 的 CHO 细胞的演化时候线。阅读有关使 CHO 细胞很是合用于细胞株开辟的特征的信息。

  • 迷信海报

    迷信海报

    领会简略的一步式杂交瘤细胞遴选性排泄检测,该检测用于在短短 8 天内(从与......融会起头)检测并分手排泄 IgG 的抗原特同性杂交瘤细胞克隆

  • 迷信海报

    迷信海报

    下载此 PDF 文件,领会有关操纵全主动克隆遴选体系疾速追踪细胞株开辟进程的信息。

  • 迷信海报

    迷信海报

    领会若何操纵 ClonePix 2 体系来遴选高程度抒发 GPCR 的细胞。

  • 迷信海报

    迷信海报

    下载 PDF 以领会开辟无血清 CHO 细胞株的进程。

  • 操纵申明

    操纵文章

    Rapid and efficient selection of high producing mammalian cells secreting non-mAb proteins

    疾速且高效地遴选可排泄非单克隆抗体卵白的高产哺乳植物细胞

    ClonePix 体系中的手艺消弭了对持续浓缩或细胞分选手艺的须要。它许可高排泄细胞的单克隆群成立在…

    Read Application Note

    阅读操纵申明

  • 操纵申明

    操纵文章

    Using ClonePix System to assess monoclonality

    操纵 ClonePix 体系评价单克隆性

    ClonePix 体系的开辟是为了降服在遴选和遴选排泄细胞进程中凡是与分手遴选的哺乳植物细胞克隆有关的良多错误谬误…

    Read Application Note

    阅读操纵申明

  • 操纵申明

    操纵文章

    Rapid automated selection of mammalian cell colonies by cell surface protein expression

    基于细胞膜卵白抒发量疾速主动遴选哺乳植物细胞克隆

    按照外表卵白抒发遴选细胞的才能在成立抒发用于下流表型分型/基因分型的方针受体的细胞系时很是有用…

    Read Application Note

    阅读操纵申明

  • 操纵申明

    操纵文章

    Rapid selection and development of GPCR expressing mammalian cell lines using novel ClonePix Technology

    就细胞株开辟而言,从转染后的浩繁细胞中发明和遴选高抒发 GPCR 克隆很是具备挑衅性。ClonePix 2 体系中的手艺…

    Read Application Note

  • 操纵申明

    操纵文章

    Enhanced development of virus-specific hybridomas using ClonePix and CloneSelect Imager Technologies

    ClonePix 体系用于从亲代杂交瘤细胞中高通量遴选数以百计的亚克隆(一向以来,亲代细胞系产量均少于…

    Read Application Note

  • 文献

    文献

    在本文中,咱们描写了一种可在细胞株开辟 (CLD) 流程初期靠得住辨认高产细胞的两重遴选方式。起首,操纵 ClonePix FL 分手滴度较高的单克隆抗体抒发…

  • 文献

    文献

    出于出产方针而发生抒发医治性单克隆抗体 (mAbs) 的不变细胞株是一个耗时且吃力的进程。

  • 文献

    文献

    比来,一些卫生政府已请求各申办公司供给有关用于发生重组 DNA (rDNA…出产细胞株的理论的具体信息。

报告内容
视频和收集钻研会
开辟和成立不变细胞系

不变的细胞系开辟任务流程

杂交瘤任务流程

杂交瘤任务流程

排泄非单克隆抗体卵白的哺乳植物细胞任务流程

遴选高产排泄非单克隆抗体卵白的哺乳植物细胞任务流程 - SynBioBeta 闪电报告

敏捷辨认中和抗体

优化的任务流程可用于敏捷辨别中和抗体与病毒颗粒

G 卵白偶联受体细胞系遴选

操纵 ClonePix 2 来辨认和遴选 GPCR 细胞系

ClonePix 2 高通量细胞克隆遴选体系

ClonePix 2

  • Number of Citations*: 347

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Dec 01, 2017
    Publication Name: Journal of Immunological Methods

    Screening and characterization of cell lines for stable production are critical tasks in identifying suitable recombinant cell lines for the manufacture of protein therapeutics. To aid this essential function we have developed a methodology for the selection of antibody expressing cells using fluorescence based ClonePix FL colony isolation and… View more

    Screening and characterization of cell lines for stable production are critical tasks in identifying suitable recombinant cell lines for the manufacture of protein therapeutics. To aid this essential function we have developed a methodology for the selection of antibody expressing cells using fluorescence based ClonePix FL colony isolation and flow cytometry analysis following intracellular staining for immunoglobulin G (IgG). Our data show that characterization of cells by flow cytometry early in the clone selection process enables the identification of cell lines with the potential for high productivity and helps to eliminate unstable cell lines. We further demonstrate a correlation between specific productivity (qP) and intracellular heavy chain (HC) content with final productivity. The unique combination of screening using ClonePix FL and the flow cytometry approaches facilitated more efficient isolation of clonal cell lines with high productivity within a 15 week timeline and which can be applied across NS0 and CHO host platforms. Furthermore, in this study we describe the critical parameters for the ClonePix FL colony based selection and the associated calculations to provide an assessment of the probability of monoclonality of the resulting cell lines.

    Contributors: Gargi Roya, Guillermo Miro-Quesadab, Li Zhuanga, Tom Martina, Jie Zhub, Herren Wua, Marcello Marellia, Michael A.Bowena  

  • Citation
    Dated: Dec 14, 2015
    Publication Name: 24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products

    The Holy Grail sought by all Bioprocess Cell Line Development (CLD) groups is achieving high yields from easily-cultured, robustly-growing cells in timelines measured in weeks rather than months. As the first bottleneck in process development, CLD must first birth its product for upstream and downstream groups to initiate their own reproductive… View more

    The Holy Grail sought by all Bioprocess Cell Line Development (CLD) groups is achieving high yields from easily-cultured, robustly-growing cells in timelines measured in weeks rather than months. As the first bottleneck in process development, CLD must first birth its product for upstream and downstream groups to initiate their own reproductive cycles. To facilitate shortened CLD timelines, scientists have turned to new technologies and automation platforms. Emerging high-throughput instrumentation such as Clonepix and Automated MicroBioreactors (AMBR) have been enthusiastically integrated into stable cell line generation platforms; however, application of these methodologies among users is divergent.

    Contributors: Venkata R Mangalampalli, Dyane Wycuff, Mingzhong Chen, David Berlinger, Elizabeth H Scheideman, Amritha Menon, Guilia Fabozzi, Althaf Hussain & Richard M Schwartz  

  • Citation
    Dated: May 25, 2014
    Publication Name: New Biotechnology

    Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to genetic instability, variable expression, product heterogeneity and prolonged development… View more

    Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to genetic instability, variable expression, product heterogeneity and prolonged development timelines. Inclusion of cis-acting ubiquitous chromatin opening elements (UCOE™) in mammalian expression vectors has been shown to improve productivity and facilitate high-level gene expression irrespective of the chromosomal integration site without lengthy gene amplification protocols. In this study we have used high-throughput robotic clone selection in combination with UCOE™ containing expression vectors to develop a rapid, streamlined approach for early-stage cell line development and isolation of high-expressing clones for mAb production using Chinese hamster ovary (CHO) cells. Our results demonstrate that it is possible to go from transfection to stable clones in only 4 weeks, while achieving specific productivities exceeding 20 pg/cell/day. Furthermore, we have used this approach to quickly screen several process-crucial parameters including IgG subtype, enhancer-promoter combination and UCOE™ length. The use of UCOE™-containing vectors in combination with automated robotic selection provides a rapid method for the selection of stable, high-expressing clones.

    Contributors: Jeff Jia Cheng Hou, Ben S. Hughes, Matthew Smede, Kar Man Leung, Kara Levine, Susan Rigby, Peter P. Gray, Trent P.Munro  

ClonePix 2 哺乳植物细胞克隆遴选体系的相干产物和办事