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撑持明场、荧光和数字共聚焦成像的主动化细胞显微成像阐发体系

ImageXpress® Pico 主动化细胞成像阐发体系不只是一台数字显微镜,还连系了高分辩率成像及壮大的阐发功效。不管是停止荧光成像仍是明场检测,该主动成像装备都具备针对细胞检测的一系列周全的预置打算,可延长进修时候,让您能够或许或许或许或许或许或许疾速起头停止尝试。凭仗数字共聚焦* 2D on-the- fly 反卷积、及时预览和多波长细胞分类等功效,ImageXpress Pico 用一个小型实惠的成像仪来加快您的迷信钻研。

  • 疾速启动

    疾速启动

    借助图标驱动的用户友爱型 CellReporterXpress® 图象收罗和阐发软件,您尝试室中的一切人都能把握这台数字显微镜。接管较短时候的培训便可起头收罗和阐发图象。

  • 操纵普遍,不只限于细胞计数

    操纵普遍,不只限于细胞计数

    操纵跨越 25 种颠末优化的预置模板,可用于多种细胞尝试,包罗细胞凋亡、线粒体评价、3D 细胞模子、活细胞/延时成像、多波长细胞分类和神经突追踪等。

  • 实惠的主动化成像阐发体系

    实惠的主动化成像阐发体系

    削减去中间尝试室/平台停止样本检测的便利。其合适的价钱能够或许或许或许或许或许让钻研职员在本身尝试室任务台上轻松停止主动成像和阐发。具备数字共聚焦、情况节制和 z-stack 收罗等挑选,您能够或许或许或许或许或许定制该体系的功效以知足您的迷信钻研。

ImageXpress Pico 主动成像

ImageXpress Pico 主动化成像体系假造阅读

特色

  • 多种成像情势

    多种成像情势

    ImageXpress Pico 体系供给 4x 到 63x 物镜,并且能够或许或许或许或许或许在黑色、明场、荧光或数字共聚焦 2D on-the-fly 反卷积成像情势下操纵。

  • 预置的阐发打算

    预置的阐发打算

    跨越 25 种预置的阐发打算,从简略的细胞计数到庞杂的神经突追踪阐发。具备 click-to-find 点击查找东西等功效,只要点击合适详细规范的少许细胞便可优化阐发参数。

  • 孔板到单个细胞的视图情势

    孔板到单个细胞的视图情势

    从孔板概览到单个细胞,可多层面显现数据。从微孔板至细胞级别的多种数据显现东西让用户能够或许或许或许或许或许或许从他们的图象和检测中领会尽能够或许或许或许或很多的信息。

  • Z-stack 成像情势

    Z-stack 成像情势

    操纵 z-stack 收罗可天生更清楚的图象,从而实现加倍切确的细胞朋分。在差别核心收罗到的一系列图象要比在一层上收罗到的图象包罗更多的细节信息。用户可在终究投影中包罗一切层面或挑选要包罗哪些层面。

  • 疾速并轻松肯定方针地区

    疾速并轻松肯定方针地区

    及时预览可便利方针成像地区的肯定,让用户阅读样品和操纵假造支配杆交互调剂核心的地位,从而节流时候和精神。

  • 情况节制

    情况节制

    操纵能够或许或许或许或许或许对湿度、CO2 和 O2 停止节制的机载情况体系来停止持续多日的延时活细胞检测。该软件经优化后可防止 z 漂移,并且能够或许或许或许或许或许或许供给对情况状况的及时监测,从而确保优化的检测前提。

休会 ImageXpress Pico 和 CellReporterXpress 的壮大组合

 

 

 

 

 

经由历程机器臂、培育箱和软件供给量身定制的尝试室主动化处置打算

 

钻研情况正在不时发生变更,现今的迷信家须要简化的长途拜候手艺和更壮大的尝试室主动化手艺。连系操纵 ImageXpress® Pico 主动化细胞成像体系与来自 PAA 的 S-LAB™ 板处置器和来自 Inheco* 的 SCILA 培育箱时,可同时实现高产量、低本钱和分歧性。

 

 

 

 

 

检查正在停止中的 ImageXpress Pico主动化 任务流程

 

 

  • 产物手册

    彩页

    除成像以外,ImageXpress® Pico 体系还可供给壮大的阐发才能,简化您的阐发流程。

ImageXpress Pico 主动化细胞成像阐发体系的操纵

  • 凋亡阐发

    凋亡阐发

    细胞凋亡是一个法式性细胞灭亡的历程,它对包罗胚胎发育和一般构造保护在内的很多生物学历程都很是主要。细胞凋亡调控的很是触及到包罗癌症在内的多种疾病。生化事务致使细胞形状特点发生变更,如细胞缩短、核断裂、染色质凝集和 mRNA 衰减,并终究致使细胞灭亡。内在或内在路子都能够或许或许或许或许或许启动细胞凋亡,并且两种路子都经由历程激活caspase酶来引诱细胞灭亡。

    领会更多  

    癌症钻研

    癌症钻研

    癌症钻研职员须要更好的钻研东西,使他们能够或许或许或许或许或许或许更轻易地钻研庞杂的、待摸索的癌细胞与其情况之间的彼此感化,并肯定癌症医治干涉干与点。用于癌症钻研的仪器和软件,在很多情况下均接纳具备生物相干性的 3D 细胞模子,如可摹拟肿瘤或器官体内情况的细胞球、类器官和器官芯片体系。

    领会更多  

  • 心肌细胞

    心肌细胞

    干细胞分解为心肌细胞后,该模子被用在开端挑选药物库中具备潜伏毒性感化的药物中,从而有助于防止投入因为毒性缘由致使在临床尝试中失利的药物而构成的华侈。

    细胞计数

    细胞增殖

    细胞计数是很多生物学尝试的根本和关头。停止药归天合物毒性、细胞增殖和细胞割裂按捺等检测时须要评价每一个孔中细胞的数目或密度。主动成像能够或许或许或许或许或许大大加快细胞计数历程,同时削减野生休息和报酬毛病。能够或许或许或许或许或许操纵多种方式停止细胞计数,比方在透射光下的非标记细胞计数或接纳荧光成像手艺停止细胞计数检测。

    领会更多  

  • COVID-19 和沾抱病钻研

    COVID-19 和沾抱病钻研

    在这里,咱们先容了沾染性疾病钻研中的罕见操纵,包罗细胞系开辟、连系亲和力、病毒中和、病毒效价等,重点存眷领会 SARS-CoV-2 病毒,以便开辟 COVID-19 的潜伏医治方式,包罗疫苗、医治方式和诊断方式。

    领会更多 

    数字共聚焦

    数字共聚焦

    细胞经由历程高度受控的旌旗灯号转导通路调理其顺应情况的才能。在很多情况下,激素或细胞因子等胞外旌旗灯号启动这些通路会致使从细胞质到细胞核的卵白转位。一旦这些卵白处于细胞核中,它们就能够或许调控靶基因的抒发。须要精准辨认差别细胞区室内的亚细胞布局或份子的检测可操纵图象反卷积功效以改良对离焦光所致使的潜伏高背景下的特定荧光旌旗灯号的辨认。撑持数字共聚焦 2D on-the-fly 反卷积的 ImageXpress Pico 体系能够或许或许或许或许或许或许检测从细胞质到细胞核的卵白转位,如操纵 TNF-α 停止处置时显现的 NF-κB 转位。图象分辩率的进步和源自图象反卷积的定量信息的保管会使核转位阐发具备更大的统计学意思。

    领会更多  

  • 活细胞成像

    活细胞成像

    活细胞成像是经由历程显微察看钻研活细胞的细胞布局和功效的钻研方式。它能够或许或许或许或许或许及时显现和定量细胞的静态变更历程。活细胞成像可操纵在多种钻研标的目的和生物操纵 ——不管是对活细胞停止持久能源学监测,仍是停止荧光标记检测。

    领会更多  

    神经突发展/神经突追踪

    神经突发展

    神经元经由历程“崛起”的细胞体延长来成立毗连。今生物景象被称为神经突发展。领会增进神经突发展的旌旗灯号传导机制可为神经毒性反映、化合物挑选和诠释影响神经再生的身分供给有代价的信息。搭配操纵 ImageXpress Micro 高内在成像阐发体系与 MetaXpress 成像阐发软件能够或许或许或许或许或许或许主动化实现基于玻片或微孔板的神经突发展的成像和阐发。

    领会更多  

  • 干细胞钻研

    干细胞钻研

    多能干细胞可用于发育生物学中的钻研或分解作为器官特同性细胞来历,在玻片或多孔板上停止活细胞或牢固细胞检测。从追踪分解到品质节制再到测定特定细胞范例功效,ImageXpress 体系能够或许或许或许或许或许助力干细胞钻研职员的一切任务流程。

    领会更多信息 

ImageXpress Pico 小我型高内在主动化细胞成像阐发体系的手艺参数

ImageXpress Pico 主动化细胞成像阐发体系的资本

报告内容
视频和收集钻研会
主动 ImageXpress Pico 任务流程

操纵 ImageXpress Pico 体系停止主动细胞成像

抗原/免疫原发明和优化

免疫学和疫苗开辟任务流程

杂交瘤任务流程

杂交瘤任务流程

ImageXpress Pico 情况节制装备

若何装置 ImageXpress Pico 情况节制体系

操纵主动成像加快您的挑选

操纵高内在和主动成像加快您的挑选

微孔板检测

经由历程基于微孔板的检测和高通量挑选加快对病毒沾染和医治的钻研

ImageXpress Pico 主动成像

ImageXpress Pico 主动化成像体系假造阅读

磁性 3D

磁性 3D 生物打印,在 2D 任务流程中停止 3D 细胞培育

开辟高通量器官芯片构造

操纵高内在成像开辟高通量器官芯片构造模子用于药物发明

Automated imaging
主动成像

主动成像与您 – 合用于每一个尝试室的定量显微镜,一切尝试室都可发生壮大数据

Imagexpress Pico 预览

若何操纵 ImageXpress Pico 及时预览功效轻松成像玻片和方针地区

规范宽场成像

ImageXpress Pico 主动细胞成像体系 – 功效更新

产物司理面谈

产物司理先容 ImageXpress Pico

ImageXpress Pico 中的情况节制设置

在 ImageXpress Pico 上设置装备摆设情况节制设置

操纵 CellReporterXpress 收罗 z 重叠图象

若何操纵 CellReporterXpress停止z-stack图象取得

ImageXpress Pico 体系

操纵主动成像,从细胞图象到得出成果仅需几分钟

设置收罗和阐发

在 ImageXpress Pico 上停止收罗和阐发设置

透射光细胞分类

在 ImageXpress Pico 上停止透射光细胞成像阐发

在 ImageXpress Pico 上考核尝试

在 ImageXpress Pico 上检查已实现的尝试

ImageXpress Pico 小我型高内在主动化细胞成像阐发体系-互动演示

ImageXpress Pico 主动化细胞成像阐发体系

ImageXpress Pico 主动化细胞成像阐发体系

  • Number of Citations*: 63

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Nov 27, 2020
    Publication Name: Human Vaccines & Immunotherapeutics 

    Lyme disease is the most common vector-borne disease in North America. The etiological agent is the spirochete Borreliella burgdorferi, transmitted to mammalian hosts by the Ixodes tick. In recent years there has been an increase in the number of cases of Lyme disease. Currently, there is no vaccine on the market for human use. We describe the… View more

    Lyme disease is the most common vector-borne disease in North America. The etiological agent is the spirochete Borreliella burgdorferi, transmitted to mammalian hosts by the Ixodes tick. In recent years there has been an increase in the number of cases of Lyme disease. Currently, there is no vaccine on the market for human use. We describe the development of a novel synthetically engineered DNA vaccine, pLD1 targeting the outer-surface protein A (OspA) of Borreliella burgdorferi. Immunization of C3 H/HeN mice with pLD1 elicits robust humoral and cellular immune responses that confer complete protection against a live Borreliella burgdorferi bacterial challenge. We also assessed intradermal (ID) delivery of pLD1 in Hartley guinea pigs, demonstrating the induction of robust and durable humoral immunity that lasts at least 1 year. We provide evidence of the potency of pLD1 by showing that antibodies targeting the OspA epitopes which have been associated with protection are prominently raised in the immunized guinea pigs. The described study provides the basis for the advancement of pDL1 as a potential vaccine for Lyme disease control.

    Contributors: Ghiabe H. Guibinga , Bikash Sahay , Heather Brown , Neil Cooch , Jing Chen , Jian Yan , Charles Reed , Meerambika Mishra , Bryan Yung , Holly Pugh , Katherine Schultheis , Rianne N. Esquivel , David B. Weiner , Laurent H. Humeau , Kate E. Broderick  & Trevor R.F. Smith 
     

  • Citation
    Dated: Nov 27, 2020
    Publication Name: Frontiers in Immunology

    Human lung fibroblasts (HLFs) treated with the viral mimetic polyinosine-polycytidylic acid (poly I:C) form an extracellular matrix (ECM) enriched in hyaluronan (HA) that avidly binds monocytes and lymphocytes. Mast cells are important innate immune cells in both asthma and acute respiratory infections including respiratory syncytial virus (RSV);… View more

    Human lung fibroblasts (HLFs) treated with the viral mimetic polyinosine-polycytidylic acid (poly I:C) form an extracellular matrix (ECM) enriched in hyaluronan (HA) that avidly binds monocytes and lymphocytes. Mast cells are important innate immune cells in both asthma and acute respiratory infections including respiratory syncytial virus (RSV); however, the effect of RSV on HA dependent mast cell adhesion and/or function is unknown. To determine if RSV infection of HLFs leads to the formation of a HA-enriched ECM that binds and enhances mast cell activity primary HLFs were infected with RSV for 48 h prior to leukocyte binding studies using a fluorescently labeled human mast cell line (LUVA). Parallel HLFs were harvested for characterization of HA production by ELISA and size exclusion chromatography. In separate experiments, HLFs were infected as above for 48 h prior to adding LUVA cells to HLF wells. Co-cultures were incubated for 48 h at which point media and cell pellets were collected for analysis. The role of the hyaladherin tumor necrosis factor-stimulated gene 6 (TSG-6) was also assessed using siRNA knockdown. RSV infection of primary HLFs for 48 h enhanced HA-dependent LUVA binding assessed by quantitative fluorescent microscopy. This coincided with increased HLF HA synthase (HAS) 2 and HAS3 expression and decreased hyaluronidase (HYAL) 2 expression leading to increased HA accumulation in the HLF cell layer and the presence of larger HA fragments. Separately, LUVAs co-cultured with RSV-infected HLFs for 48 h displayed enhanced production of the mast cell proteases, chymase, and tryptase. Pre-treatment with the HA inhibitor 4-methylumbelliferone (4-MU) and neutralizing antibodies to CD44 (HA receptor) decreased mast cell protease expression in co-cultured LUVAs implicating a direct role for HA. TSG-6 expression was increased over the 48-h infection. Inhibition of HLF TSG-6 expression by siRNA knockdown led to decreased LUVA binding suggesting an important role for this hyaladherin for LUVA adhesion in the setting of RSV infection. In summary, RSV infection of HLFs contributes to inflammation via HA-dependent mechanisms that enhance mast cell binding as well as mast cell protease expression via direct interactions with the ECM.

    Contributors: Stephen R. Reeves, Kaitlyn A. Barrow, Lucille M. Rich, Maria P. White, Nicholas J. Shubin, Christina K. Chan, Inkyung Kang, Steven F. Ziegler, Adrian M. Piliponsky, Thomas N. Wight, and Jason S. Debley
     

  • Citation
    Dated: May 20, 2020
    Publication Name: Nature

    The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for the pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of the disease. We have previously engineered a synthetic DNA vaccine targeting the MERS coronavirus Spike (S) protein, the major surface… View more

    The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for the pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of the disease. We have previously engineered a synthetic DNA vaccine targeting the MERS coronavirus Spike (S) protein, the major surface antigen of coronaviruses, which is currently in clinical study. Here we build on this prior experience to generate a synthetic DNA-based vaccine candidate targeting SARS-CoV-2 S protein. The engineered construct, INO-4800, results in robust expression of the S protein in vitro. Following immunization of mice and guinea pigs with INO-4800 we measure antigen-specific T cell responses, functional antibodies which neutralize the SARS-CoV-2 infection and block Spike protein binding to the ACE2 receptor, and biodistribution of SARS-CoV-2 targeting antibodies to the lungs. This preliminary dataset identifies INO-4800 as a potential COVID-19 vaccine candidate, supporting further translational study.

    Contributors: Trevor R. F. Smith, Ami Patel, Stephanie Ramos, Dustin Elwood, Xizhou Zhu, Jian Yan, Ebony N. Gary, Susanne N. Walker, Katherine Schultheis, Mansi Purwar, Ziyang Xu, Jewell Walters, Pratik Bhojnagarwala, Maria Yang, Neethu Chokkalingam, Patrick Pezzoli, Elizabeth Parzych, Emma L. Reuschel, Arthur Doan, Nicholas Tursi, Miguel Vasquez, Jihae Choi, Edgar Tello-Ruiz, Igor Maricic, Mamadou A. Bah, Yuanhan Wu, Dinah Amante, Daniel H. Park, Yaya Dia, Ali Raza Ali, Faraz I. Zaidi, Alison Generotti, Kevin Y. Kim, Timothy A. Herring, Sophia Reeder, Viviane M. Andrade, Karen Buttigieg, Gan Zhao, Jiun-Ming Wu, Dan Li, Linlin Bao, Jiangning Liu, Wei Deng, Chuan Qin, Ami Shah Brown, Makan Khoshnejad, Nianshuang Wang, Jacqueline Chu, Daniel Wrapp, Jason S. McLellan, Kar Muthumani, Bin Wang, Miles W. Carroll, J. Joseph Kim, Jean Boyer, Daniel W. Kulp, Laurent M. P. F. Humeau, David B. Weiner & Kate E. Broderick
     

ImageXpress Pico 主动化细胞成像阐发体系的相干产物和办事