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高内在共聚焦成像处置打算供给水镜设置装备摆设

ImageXpress® Micro Confocal 体系是一个高内在处置打算,可在安稳细胞和活细胞的宽场和共聚焦成像之间一键切换。它可捕获完全生物体、厚构造、2D 和 3D 模子和细胞或细胞内事务的高品质图象。转盘式共聚焦和 sCMOS 相机可对心肌细胞搏动和干细胞分解等疾速和罕有事务停止成像。因为具备 MetaXpress 软件且有水镜等矫捷设置装备摆设可供挑选,该体系可撑持从 3D 尝试开辟到细胞挑选的多种共聚焦成像操纵。

  • 取得更高品质的图象

    取得更高品质的图象

    操纵咱们专有的 AgileOptix™ 转盘式共聚焦手艺、宽视线和高亮度光源来捕获对照度极佳的高分辩率图象。

  • 自界说图象收罗和阐发

    自界说图象收罗和阐发

    对收罗和阐发参数停止终究节制,从而完成从 3D 布局阐发到生物体或细胞群内特定方针的靶向成像的多种操纵。操纵水镜、情况节制等规范设置装备摆设或咱们的其余高机能定制化组件来停止更多操纵。

  • 在更短的时候内阐发更多的数据

    在更短的时候内阐发更多的数据

    MetaXpress® PowerCore™ 软件可加速高通量情况下的阐发速率。该软件将图象处置使命分派最多 CPU 情况。

 ImageXpress Micro Confocal 假造阅读

ImageXpress Micro Confocal 假造阅读

特色

  • 宽静态规模

    经由历程静态规模 > 3log 的光强检测才能来定量单张图象中的低强度和高强度旌旗灯号。

  • 专有 AgileOptix 转盘式共聚焦

    该手艺可经由历程特地设想的光学器件、高机能固态光学引擎和 sCMOS 手艺来前进活络度。可切换的转盘布局供给速率与分辩率之间的矫捷挑选。

  • 宽视线

    宽视线可完成全孔共聚焦成像并防止方针漏掉。

  • 可选机载主动液流体系

    可选机载主动液流体系合用于触及化合物增添、孔洗濯和培育基互换的检测。

  • 3D 精准测定

    MetaXpress 3D 阐发模块针对共聚焦成像停止了优化,可完成体积和间隔的 3D 丈量。

  • 多种成像情势

    该体系可供给相差和明场非标记成像、荧光、宽场、比色和共聚焦成像,和水镜成像(作为规范设置装备摆设)。

AgileOptix™ 手艺

咱们专有的 AgileOptix 手艺让 ImageXpress Micro 共聚焦体系能够或许或许或许或许或许供给高请求操纵所需的活络度和通量。AgileOptix 是壮大的固态光引擎、出格设想的光学器件、科研级 CMOS 传感器与可变布局式转盘的组合。

AgileOptix 手艺合用于 ImageXpress Micro 共聚焦体系

 

 

 

 

MetaXpress 3D 阐发东西套件简介

MetaXpress 高内在图象收罗和阐发软件

 

  • 操纵可扩大的流线型使命流程,合适高通量请求
  • 调试您的阐发东西以处置 3D 阐发等有挑衅的情况
  • 停止第三方硬件和宁静数据库之间的主动数据传输
  • 操纵 MetaXpress 软件模块设置数百种惯例操纵的高内在挑选 (HCS) 检测打算

 

 

 

 

细胞图象库

Micro 共聚焦高内在成像体系
ImageXpress 高内在成像处置打算
ImageXpress Micro 共聚焦成像体系
ImageXpress Micro 成像体系
Micro 共聚焦成像体系
 
在开辟时代操纵客户样品取得的数据和图象。成果能够或许或许或许或许会有所差别。*凸起功效的价钱、托付时候和规格将按照彼此协议的手艺请求而变更。能够或许或许或许或许会按照处置打算请求调剂规范机能。

 

 

  • 宣扬页

    宣扬页

    操纵 ImageXpress® Micro Confocal 体系获更深的 3D 和厚构造样品的更大都据,可扩大、高机能的高内在挑选处置打算。

ImageXpress Micro Confocal 共聚焦高内在成像阐发体系的操纵

ImageXpress Micro Confocal 转盘共聚焦高内在成像阐发体系的手艺参数和可选设置装备摆设

ImageXpress Micro Confocal 转盘共聚焦高内在成像阐发体系的资本

报告内容
视频和收集钻研会
抗原/免疫原发明和优化

免疫学和疫苗开辟使命流程

杂交瘤使命流程

杂交瘤使命流程

操纵 3D 成像的主动类器官检测

21 世纪的疾病建模:操纵 3D 成像的主动类器官检测

器官芯片体系合用于药物发明和疾病建模

合用于药物发明和疾病建模的高通量、类器官源性器官芯片体系

开释 Cell Painting 的气力

开释 Cell Painting 的气力

3D 细胞培育

3D 细胞培育、构造断根和高内在成像,以追求有用的非酒精性脂肪性肝病 (NAFLD) 处置打算

从高内在检测过渡到 3D 检测

从高内在检测过渡到 3D 检测:迷信机缘和成像挑衅

水浸物镜和高内在成像

操纵水浸物镜更深切领会细胞 3D 布局

基于 AI 的表型判定方式

用于停止人引诱多能性干细胞 (iPSC) 源性神经元细胞高内在表型判定的一种基于野生智能 (AI) 的方式

实行 3D 神经元细胞球

在药物发明中操纵 3D 神经元细胞球

操纵主动成像加速您的挑选

操纵高内在和主动成像加速您的挑选

微孔板检测

经由历程基于微孔板的检测和高通量挑选加速对病毒沾染和医治的钻研

 ImageXpress Micro Confocal 假造阅读

ImageXpress Micro Confocal 假造阅读

磁性 3D

磁性 3D 生物打印,在 2D 使命流程中停止 3D 细胞培育

Labtube 合适尝试室主动化与挑选协会 (SLAS) 请求

LabTube 与 Molecular Devices 和 MIMETAS 会晤(Susan Murphy 和 Sebastiaan Trietsch)

操纵 MetaXpress 停止微孔板标注和曲线拟合

操纵 MetaXpress 停止微孔板标注和曲线拟合

在 ImageXpress Micro Confocal 上操纵 MetaXpress 停止微孔板收罗

疾速入门指南:在 ImageXpress Micro Confocal 上操纵 MetaXpress 停止微孔板收罗

在 ImageXpress Micro Confocal 上操纵 MetaXpress 检查图象

疾速入门指南:在 ImageXpress Micro Confocal 上操纵 MetaXpress 检查图象

在 ImageXpress Micro Confocal 上操纵 MetaXpress 停止图象阐发

在 ImageXpress Micro Confocal 上操纵 MetaXpress 停止从图象收罗到阐发的根基使命流程

开辟高通量器官芯片构造

操纵高内在成像开辟高通量器官芯片构造模子用于药物发明

iPSC 源性心肌细胞和神经元细胞球

iPSC 源性心肌细胞和神经元细胞球的毒性钻研

3D 体外模子

优化用于心理相干性 3D 体外模子的高内在挑选东西

微流控平台中 3D 神经元收集的外形表征

微流控平台中 3D 神经元收集的外形表征

肿瘤细胞球的 3D 成像

肿瘤细胞球的 3D 成像

用于辨认 miRNA 的高内在挑选

用于辨认 miRNA 的高内在挑选

STAT3 旌旗灯号通路的挑选性按捺剂的辨别

STAT3 旌旗灯号通路的挑选性按捺剂的辨别

Oliver Kepp 和 Jayne Hesley - 操纵高内在成像检测细胞灭亡旌旗灯号

Oliver Kepp 和 Jayne Hesley - 癌症的特点 - 操纵高内在成像检测并定量细胞灭亡旌旗灯号

ImageXpress Micro 多维共聚焦体系

经由历程新 ImageXpress Micro 共聚焦体系可停止多维高通量成像

拓展高内在挑选的操纵规模

拓展高内在挑选的操纵规模

为全基因组 RNAi 挑选筹办检测

操纵高内在成像阐发体系停止全基因组 RNAi 挑选检测

多重高内在肝脏毒性检测

操纵 iPSC 源性肝细胞停止多重高内在肝脏毒性检测

操纵体外构造模子对细胞层外形产生停止高内在成像阐发

操纵体外构造模子对细胞层外形产生停止高内在成像阐发

简略、矫捷的庞杂生物事务高内在成像

可完成庞杂生物事务定量的简略、矫捷的高内在成像

古代主动化和高内在成像东西

用于挑选干细胞源性心肌细胞的古代主动化和高内在成像东西

从亚细胞布局到细胞球的 3D 成像阐发

从亚细胞布局到细胞球,对样品停止高通量 3D 成像阐发

活细胞成像可用于钻研细胞割裂时候

操纵活细胞成像功效对细胞割裂时候的调控机制停止钻研

高通量 RNA 挑选可用于辨认宿主因子

操纵高通量 RNA 挑选以辨认影响病毒沾染的宿主因子

用于发明抗体药物的 HCA 东西的操纵

用于发明抗体药物的 HCA 东西的操纵

操纵高内在成像停止 3D 细胞球检测

操纵高内在成像设置 3D 细胞球检测

操纵高通量芯片器官 (Organ-on-a-Chip) 平台的心理学相干构造模子

操纵高通量器官芯片平台的心理相干性构造模子

多无能细胞在药物发明中的操纵

新兴引诱性多功效干细胞在药物发明中的操纵

StemoniX microBrain 3D 检测

合用于高通量挑选 (HTS) 的 StemoniX microBrain 3D 即用检测板

  • Number of Citations*: 1,530

    Latest Citations: For a complete list, please .

    *Source:

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Upstate Medical University

    All eukaryotic cells tightly control cellular pH. Proper control of cytoplasmic pH is essential for normal metabolism and cell growth, and acidification of organelles such as the lysosome, endosome, and Golgi apparatus is essential for protein sorting and degradation, ion homeostasis, and signal transduction. The vacuolar ATPase (V-ATPase) is one… View more

    All eukaryotic cells tightly control cellular pH. Proper control of cytoplasmic pH is essential for normal metabolism and cell growth, and acidification of organelles such as the lysosome, endosome, and Golgi apparatus is essential for protein sorting and degradation, ion homeostasis, and signal transduction. The vacuolar ATPase (V-ATPase) is one of the central players in pH control. All eukaryotic cells have V-ATPases of remarkably similar structure, and loss of V-ATPase function is lethal at early stages of development in higher eukaryotes and conditionally lethal in fungi.

    Contributors: Patricia Kane, PhD  

  • Citation
    Dated: Jan 01, 2019
    Publication Name: Toxicology Science

    Neurological disorders affect millions of people worldwide and appear to be on the rise. Whereas the reason for this increase remains unknown, environmental factors are a suspected contributor. Hence, there is an urgent need to develop more complex, biologically relevant, and predictive in vitro assays to screen larger sets of compounds with the… View more

    Neurological disorders affect millions of people worldwide and appear to be on the rise. Whereas the reason for this increase remains unknown, environmental factors are a suspected contributor. Hence, there is an urgent need to develop more complex, biologically relevant, and predictive in vitro assays to screen larger sets of compounds with the potential for neurotoxicity. Here, we employed a human induced pluripotent stem cell (iPSC)-based 3D neural platform composed of mature cortical neurons and astrocytes as a model for this purpose. The iPSC-derived human 3D cortical neuron/astrocyte co-cultures (3D neural cultures) present spontaneous synchronized, readily detectable calcium oscillations. This advanced neural platform was optimized for high-throughput screening in 384-well plates and displays highly consistent, functional performance across different wells and plates. Characterization of oscillation profiles in 3D neural cultures was performed through multi-parametric analysis that included the calcium oscillation rate and peak width, amplitude, and waveform irregularities. Cellular and mitochondrial toxicity were assessed by high-content imaging. For assay characterization, we used a set of neuromodulators with known mechanisms of action. We then explored the neurotoxic profile of a library of 87 compounds that included pharmaceutical drugs, pesticides, flame retardants, and other chemicals. Our results demonstrated that 57% of the tested compounds exhibited effects in the assay. The compounds were then ranked according to their effective concentrations based on in vitro activity. Our results show that a human iPSC-derived 3D neural culture assay platform is a promising biologically relevant tool to assess the neurotoxic potential of drugs and environmental toxicants.

    Contributors: Oksana Sirenko 1 , Frederick Parham 2 , Steven Dea 3 , Neha Sodhi 3 , Steven Biesmans 3 , Sergio Mora-Castilla 3 , Kristen Ryan 2 , Mamta Behl 2 , Grischa Chandy 1 , Carole Crittenden 1 , Sarah Vargas-Hurlston 1 , Oivin Guicherit 3 , Ryan Gordon 3 , Fabian Zanella 3 , Cassiano Carromeu  

  • Citation
    Dated: Sep 01, 2018
    Publication Name: Assay and Drug Development Technologies

    This study set out to develop a high-throughput multiplexed assay using iPSC-derived skeletal myoblasts that can be used as a first-pass screen to assess the potential for chemicals to affect skeletal muscle. We found that cytotoxicity and cytoskeletal integrity are most useful and reproducible assays for the skeletal myoblasts when evaluating… View more

    This study set out to develop a high-throughput multiplexed assay using iPSC-derived skeletal myoblasts that can be used as a first-pass screen to assess the potential for chemicals to affect skeletal muscle. We found that cytotoxicity and cytoskeletal integrity are most useful and reproducible assays for the skeletal myoblasts when evaluating overall cellular health or gauging disruptions in actin polymerization following 24 h of exposure. Both assays are based on high-content imaging and quantitative image processing to derive quantitative phenotypes. Both assays showed good to excellent assay robustness and reproducibility measured by interplate and interday replicability, coefficients of variation of negative controls, and Z′-factors for positive control chemicals. Concentration response assessment of muscle-related toxicants showed specificity of the observed effects compared to the general cytotoxicity. Overall, this study establishes a high-throughput multiplexed assay using skeletal myoblasts that may be used for screening and prioritization of chemicals for more complex tissue chip-based and in vivo evaluation.

    Contributors: William D. Klaren and Ivan Rusyn  

  • Citation
    Dated: Aug 01, 2017
    Publication Name: Assay and Drug Development Technologies

    Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a major challenge in drug discovery. The focus of this study was to establish high-throughput compatible three-dimensional (3D) cardiotoxicity assays using human induced pluripotent stem cell-derived cardiomyocytes. Using both high-… View more

    Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a major challenge in drug discovery. The focus of this study was to establish high-throughput compatible three-dimensional (3D) cardiotoxicity assays using human induced pluripotent stem cell-derived cardiomyocytes. Using both high-content imaging and fast kinetic fluorescence imaging, the impact of various compounds on the beating rates and patterns of cardiac spheroids was monitored by changes in intracellular Ca2+ levels with calcium-sensitive dyes. Advanced image analysis methods were implemented to provide multiparametric characterization of the Ca2+ oscillation patterns. In addition, we used confocal imaging and 3D analysis methods to characterize compound effects on the morphology of 3D spheroids. This phenotypic assay allows for the characterization of parameters such as beating frequency, amplitude, peak width, rise and decay times, as well as cell viability and morphological characteristics. A set of 22 compounds, including a number of known cardioactive and cardiotoxic drugs, was assayed at different time points, and the calculated EC50 values for compound effects were compared between 3D and two-dimensional (2D) model systems. A significant concordance in the phenotypes was observed for compound effects between the two models, but essential differences in the concentration responses and time dependencies of the compound-induced effects were observed. Together, these results indicate that 3D cardiac spheroids constitute a functionally distinct biological model system from traditional flat 2D cultures.In conclusion, we have demonstrated that phenotypic assays using 3D model systems are enabled for screening and suitable for cardiotoxicity assessment in vitro.

    Contributors: Oksana Sirenko, Michael K. Hancock, Carole Crittenden, Matthew Hammer, Sean Keating, Coby B. Carlson, and Grischa Chandy  

  • Citation
    Dated: Sep 01, 2016
    Publication Name: ASSAY and Drug Development Technologies

    Cell models are becoming more complex to better mimic the in vivo environment and provide greater predictivity for compound efficacy and toxicity. There is an increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling developmental and tissue biology with the goal of accelerating translational research in these areas… View more

    Cell models are becoming more complex to better mimic the in vivo environment and provide greater predictivity for compound efficacy and toxicity. There is an increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling developmental and tissue biology with the goal of accelerating translational research in these areas. Accordingly, the development of high-throughput quantitative assays using 3D cultures is an active area of investigation. In this study, we have developed and optimized methods for the formation of 3D liver spheroids derived from human iPS cells and used those for toxicity assessment. We used confocal imaging and 3D image analysis to characterize cellular information from a 3D matrix to enable a multi-parametric comparison of different spheroid phenotypes. The assay enables characterization of compound toxicities by spheroid size (volume) and shape, cell number and spatial distribution, nuclear characterization, number and distribution of cells expressing viability, apoptosis, mitochondrial potential, and viability marker intensities. In addition, changes in the content of live, dead, and apoptotic cells as a consequence of compound exposure were characterized. We tested 48 compounds and compared induced pluripotent stem cell (iPSC)-derived hepatocytes and HepG2 cells in both two-dimensional (2D) and 3D cultures. We observed significant differences in the pharmacological effects of compounds across the two cell types and between the different culture conditions. Our results indicate that a phenotypic assay using 3D model systems formed with human iPSC-derived hepatocytes is suitable for high-throughput screening and can be used for hepatotoxicity assessment in vitro.

    Contributors: Oksana Sirenko, Michael K. Hancock, Jayne Hesley, Dihui Hong, Avrum Cohen, Jason Gentry, Coby B. Carlson, and David A. Mann  

  • Citation
    Dated: Mar 01, 2016
    Publication Name: Methods

    3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery and toxicology screening. As a result, 3D culture technologies adapted for high-throughput screening formats are prevalent. While a multitude of assays have been reported and validated for high-throughput… View more

    3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery and toxicology screening. As a result, 3D culture technologies adapted for high-throughput screening formats are prevalent. While a multitude of assays have been reported and validated for high-throughput imaging (HTI) and high-content screening (HCS) for novel drug discovery and toxicology, limited HTI/HCS with large compound libraries have been reported. Nonetheless, 3D HTI instrumentation technology is advancing and this technology is now on the verge of allowing for 3D HCS of thousands of samples. This review focuses on the state-of-the-art high-throughput imaging systems, including hardware and software, and recent literature examples of 3D organotypic culture models employing this technology for drug discovery and toxicology screening.

    Contributors: Linfeng Li, Qiong Zhou, Ty C.Voss, Kevin L., Quick, Daniel V.LaBarbera  

ImageXpress Micro Confocal 转盘共聚焦高内在成像阐发体系的相干产物和办事

操纵矫捷的高机能成像处置打算扩大您的钻研

Molecular Devices 为 ImageXpress Micro 共聚焦高内在成像体系供给矫捷的设置装备摆设,以知足您的钻研须要并轻松捕获来自差别样本范例的图象(包含悬滴培育和圆底或平底微孔板),从而在情况节制前提下监测细胞安康能源学等。凭仗 30 年以上的成像专业常识堆集,咱们能赞助您挑选准确的设置装备摆设,从而确保您的检测取得最好图象。

规范硬件选项

  • 水浸物镜

    水浸物镜

    20X、40X 和 60X 水浸物镜可前进收罗时代的多少切确度并削减光的折射,从而在较短暴光时候内取得更亮的光强度。

     

  • 相衬

    透射光源

    透射光照明立柱能够或许或许或许或许或许收罗未染色细胞的高对照度图象,使其轻松与背景辨别开。

     

  • 完全的高通量持久能源学

    情况节制

    情况节制能够或许或许或许或许或许坚持温度和湿度水平,同时最大水平削减蒸发,从而停止延续多天的活细胞延时成像。

     

  • 机器主动化

    板载机器流控手艺

    集成主动流控体系能够或许或许或许使须要增添化合物、洗孔和培育基互换的检测使命流程完成主动化。

     

 

定制选项

 

Molecular Devices 可胜利调剂 ImageXpress Micro 共聚焦高内在成像体系,以归入具备下述功效的定制软件和硬件和培育箱、液体使命站和机器人等其余尝试室组件的集成,从而完成一个全主动化的使命单位。凭仗在性命迷信范畴跨越 30 年的经历,咱们将为您供给高品质的产物和环球规模内的撑持。

发卖须遵照咱们的定制产物采办条目,概况见 www.moleculardevices.com/custom-products-purchase-terms

  • 高强度激光

    高强度激光

    操纵 5 通道或 7 通道高强度激光来扩大尝试才能。

     

  • 主动移液器

    及时剂量反映

    主动移液器可在完成 > 100 帧/秒的及时图象收罗的同时完成化合物增添。

     

  • 共聚焦磁盘模块

    深层构造穿透共聚焦转盘模块

    深层构造穿透共聚焦转盘模块可削减旌旗灯号串扰,从而前进离焦光按捺并更深地穿透进构造中。

     

  • 完全的高通量持久能源学

    完全的高通量持久能源学

    在长时候下成像多块板,同时坚持分歧的温度、O2(缺氧)、CO2 和湿度前提。扩大活细胞主动操纵才能至 200多块板。

     

  • 机器主动化

    扩大的机器主动化

    增添通量、消弭报酬偏差、坚持无菌性和完成分歧的样品处置。模块化主动化设想——可在模块中增添组件并对其停止进级。

     

 
 

 

高输入激光激起可将暴光时候削减高达 75%。†激光光源能够或许或许或许或许 5 通道或 7 通道光源的情势供给,输入强度为 400-1,000 mW/通道。7 通道激光光源包含近红外光,是具备更高多重检测请求客户的抱负之选。

  • 取得信噪比更高、更清楚的图象
  • 因为暴光时候大大延长,扫描速率可加速高达两倍
  • 操纵合用于 CFP 和 YFP 的激光运转 FRET 尝试
规范
规范强度图象
激光
高强度激光图象

 

在不异暴光下拍摄到的图象。

在对较厚的构造样本停止成像时,搭配操纵专业的深层构造穿透共聚焦转盘模块与激光光源可前进深层构造的光穿透性,从而取得更高分辩率和更清楚的图象。†

  • 改良对焦立体之外光的按捺
  • 削减恍惚(针孔交互感化)
  • 更深地穿透厚构造样品,从而取得更清楚的图象
规范转盘
规范扭转盘
深层构造穿透共聚焦转盘模块
操纵共聚焦盘停止深构造渗入

在不异暴光下拍摄到的图象。

在开辟时代操纵客户样品取得的数据和图象。成果能够或许或许或许或许会有所差别。*凸起功效的价钱、托付时候和规格将按照彼此协议的手艺请求而变更。能够或许或许或许或许会按照处置打算请求调剂规范机能。